CAJ | 학술논문

本研究采用高脂饲料诱导小鼠，建立非酒精性脂肪肝模型并分析其对脂肪细胞因子瘦素和脂联素的影响。雄性ICR小鼠12只，随机分成对照组和高脂组，每组6只，分别饲喂基础饲料和高脂饲料，于连续喂养4周和8周后，眼球采血处死，称取小鼠的体重和肝重，计算肝指数，并测定血清中的生化指标。结果显示：与对照组相比，高脂组小鼠的肝指数极显著升高（P ＜0.01），高脂组的谷丙转氨酶（ALT）、谷草转氨酶（AST）、胆固醇（TC）、葡萄糖（GLU）、碱性磷酸酶（ALP）、瘦素和胰岛素水平极显著或显著高于对照组（P＜0.01或P＜0.05），而甘油三酯的变化不明显（P>0.05），脂联素的水平极显著低于对照组（P＜0.01）。试验结果表明，高脂饲料饲喂小鼠会引起模型组小鼠发生脂肪肝变化，并产生胰岛素抵抗、瘦素抵抗和低脂联素血症。
본연구채용고지사료유도소서，건립비주정성지방간모형병분석기대지방세포인자수소화지련소적영향。웅성ICR소서12지，수궤분성대조조화고지조，매조6지，분별사위기출사료화고지사료，우련속위양4주화8주후，안구채혈처사，칭취소서적체중화간중，계산간지수，병측정혈청중적생화지표。결과현시：여대조조상비，고지조소서적간지수겁현저승고（P ＜0.01），고지조적곡병전안매（ALT）、곡초전안매（AST）、담고순（TC）、포도당（GLU）、감성린산매（ALP）、수소화이도소수평겁현저혹현저고우대조조（P＜0.01혹P＜0.05），이감유삼지적변화불명현（P>0.05），지련소적수평겁현저저우대조조（P＜0.01）。시험결과표명，고지사료사위소서회인기모형조소서발생지방간변화，병산생이도소저항、수소저항화저지련소혈증。
A non-alcoholic steatohepatitis animal model induced by high-fat diet was established ,and its effects on le ptin and adiponectin were studied.12 male ICR mice were randomly divided into 2 groups ,each group contained 6 mice.The control group was fed with standard diet and the model group fed with high-fat diet.The blood samples of two groups were collected by removal of the mouse eye after 4 weeks and 8 weeks feeding.Then the body and liver were weighted.The serum were assayed at the 4th and 8th week by automatic biochemical analyzer.Serum leptin ,adiponectin and insulin levels were measured by enzyme-linked immunosorbent assay (ELISA).The results showed that:compared with control group,the level of liver index significantly increased.The levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total cholesterol (TC),glucose (GLU),alkaline phosphatase (ALP),leptin and isulin in serum were significantly higher than those of control group(P < 0.01 or P < 0.05).Triglyceride(TG) did not change significantly (P > 0.05).Serum adiponectin level of high-fat group was significantly lower than that of control group (P < 0.01).So feeding with high-fat diet could induce steatoheoatitis in mouse rapidly and develop insulin resistance ,leptin resistance and hypoadiponectinemia.