CAJ | 학술논문

目的：探讨EB病毒（EBV）-DNA拷贝数和EBV编码的小RNA （EBERs）原位表达在鼻NK/T细胞淋巴瘤（NKTCL）是否具有一致性。方法回顾性分析50例鼻NKTCL的临床病理特征，免疫组织化学检测CD3、CD45RO、CD20、CD56、CD79a、TIA1、Granzyme B表达情况；分别运用实时定量PCR检测石蜡组织中EBV-DNA的拷贝数以及原位杂交检测EBERs，分析与EBV感染与鼻NKTCL的关系。结果鼻NKTCL大部分发生部位位于鼻腔占88％（44/50），伴有出血、坏死以及溃疡者高达100％（50/50）。CD3、CD56、TIA1、Granzyme B及EBV-DNA 和EBERs阳性表达率达100％。所有患者的EBV-DNA拷贝数均处于一个较高的水平，最小拷贝数为127.105，最大拷贝数为1450000，其中位拷贝数是23750。结论实时定量PCR检测EBV-DNA拷贝数与原位杂交技术检测EBERs表达具有高度一致性。因此鉴于鼻NKTCL具有组织形态复杂多样性的特点，在诊断鼻NKTCL时需综合考虑临床表现、形态学改变、免疫表型及EBV-DNA或EBERs高水平表达，方能明确诊断。
목적：탐토EB병독（EBV）-DNA고패수화EBV편마적소RNA （EBERs）원위표체재비NK/T세포림파류（NKTCL）시부구유일치성。방법회고성분석50례비NKTCL적림상병리특정，면역조직화학검측CD3、CD45RO、CD20、CD56、CD79a、TIA1、Granzyme B표체정황；분별운용실시정량PCR검측석사조직중EBV-DNA적고패수이급원위잡교검측EBERs，분석여EBV감염여비NKTCL적관계。결과비NKTCL대부분발생부위위우비강점88％（44/50），반유출혈、배사이급궤양자고체100％（50/50）。CD3、CD56、TIA1、Granzyme B급EBV-DNA 화EBERs양성표체솔체100％。소유환자적EBV-DNA고패수균처우일개교고적수평，최소고패수위127.105，최대고패수위1450000，기중위고패수시23750。결론실시정량PCR검측EBV-DNA고패수여원위잡교기술검측EBERs표체구유고도일치성。인차감우비NKTCL구유조직형태복잡다양성적특점，재진단비NKTCL시수종합고필림상표현、형태학개변、면역표형급EBV-DNA혹EBERs고수평표체，방능명학진단。
Objective To explore whether the EBV-DNA copy number and EBERs expression are concordant with nasal NK/T cell lymphoma (NKTCL)diagnosis. Methods 50 patients diagnosed as nasal NKTCL were investigated in this study retrospectively. Expressions of CD3,CD45RO,CD20,CD56,CD79a, TIA1 ,Granzyme B were applied with immunohistochemistry staining. EBV-encoded small RNAs (EBERs) was measured by in situ hybridization,and EBV-DNA copy numbers with real-time quantitative PCR,respec-tively. Results 88% (44/50)of the nasal NKTCL occurred in nasal cavity. All cases exhibits necrosis,ul-ceration and bleeding. Expressions of CD3,CD56,TIA1 and Granzyme B as well as high level of EBV-DNA and EBERs were observed in all cases. The median EBV-DNA copy number was shown as 23,750 (the range of 1 27.1 05 to 1 450 000). Conclusions The high consistency between EBV-DNA copy numbers and EBERs expression was confirmed. Due to its complex morphological diversity,clinical manifestation,pathological changes,and phenotyping with EBV-DNA copy numbers/EBERs expression should be appiled when nasal NK-TCL was considered.