CAJ | 학술논문

目的评价异丙酚对急性肝衰竭小鼠肝损伤的影响.方法成年雄性ICR小鼠80只,1月龄,体重20 ～ 25 g,采用随机数字表法分为3组(n=20):对照组(Ⅰ组)、急性肝衰竭组(Ⅱ组)和异丙酚组(Ⅲ组).采用腹腔注射D-氨基半乳糖和LPS的方法制备小鼠急性肝衰竭模型,Ⅲ组腹腔注射D-氨基半乳糖和LPS后6h内,每隔1h尾静脉注射异丙酚5 mg/kg,其余组相应时点注射等容量生理盐水.腹腔注射D-氨基半乳糖和LPS后1、3和6 h(T1-3)时采尾静脉血样,测定血清AST和ALT活性,ELISA法测定血清TNF-α、IL-1β和IL-10浓度；腹腔注射D-氨基半乳糖和LPS后12 h观察小鼠生存情况,采用颈椎脱臼法处死存活小鼠,取肝组织观察病理学结果.结果与Ⅰ组比较,Ⅱ组和Ⅲ组各时点血清AST和ALT活性、Ⅱ组T1,2时血清TNF-α浓度、各时点血清IL-1β和IL-10浓度,Ⅲ组T1时血清TNF-α浓度、T2,3时血清IL-1β和IL-10浓度升高(P＜0.05)；与Ⅱ组比较,Ⅲ组各时点血清AST和ALT活性、T1,2时血清TNF-α浓度、T2,3时血清IL-1β和IL-10浓度降低,腹腔注射后12 h小鼠生存率升高(P＜0.05).Ⅱ组和Ⅲ组肝组织病理学损伤程度依次减轻.结论异丙酚可通过抑制炎性反应减轻急性肝衰竭小鼠的肝损伤.
목적 평개이병분대급성간쇠갈소서간손상적영향.방법 성년웅성ICR소서80지,1월령,체중20 ～ 25 g,채용수궤수자표법분위3조(n=20):대조조(Ⅰ조)、급성간쇠갈조(Ⅱ조)화이병분조(Ⅲ조).채용복강주사D-안기반유당화LPS적방법제비소서급성간쇠갈모형,Ⅲ조복강주사D-안기반유당화LPS후6h내,매격1h미정맥주사이병분5 mg/kg,기여조상응시점주사등용량생리염수.복강주사D-안기반유당화LPS후1、3화6 h(T1-3)시채미정맥혈양,측정혈청AST화ALT활성,ELISA법측정혈청TNF-α、IL-1β화IL-10농도；복강주사D-안기반유당화LPS후12 h관찰소서생존정황,채용경추탈구법처사존활소서,취간조직관찰병이학결과.결과 여Ⅰ조비교,Ⅱ조화Ⅲ조각시점혈청AST화ALT활성、Ⅱ조T1,2시혈청TNF-α농도、각시점혈청IL-1β화IL-10농도,Ⅲ조T1시혈청TNF-α농도、T2,3시혈청IL-1β화IL-10농도승고(P＜0.05)；여Ⅱ조비교,Ⅲ조각시점혈청AST화ALT활성、T1,2시혈청TNF-α농도、T2,3시혈청IL-1β화IL-10농도강저,복강주사후12 h소서생존솔승고(P＜0.05).Ⅱ조화Ⅲ조간조직병이학손상정도의차감경.결론 이병분가통과억제염성반응감경급성간쇠갈소서적간손상.
Objective To evaluate the effect of propofol on liver injury in mice with acute liver failure (ALF).Methods Eighty adult male ICR mice,aged 1 months,weighing 20-25 g,were randomly divided into 3 groups (n =20 each) using a random number table:control group (group Ⅰ),ALF group (group Ⅱ),and ALF + propofol group (group Ⅲ).ALF model was established with intra-peritoneal D-galactosamine (D-GaIN) and lipopolysaccharide (LPS).Propofol 5 mg/kg was injected via the tail vein every 1 h within 6 h after injection of DGaIN and LPS in group Ⅲ,while the equal volume of normal saline was given instead in the other groups.Venous blood samples were taken from the tail vein at 1,3 and 6 h after injection of D-GaIN and LPS (T1-3) to detect the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and serum tumor necrosis factor-alpha (TNF-α),interleukin-1β (IL-1β) and IL-10 concentrations (by ILISA).The survival within 12 h after injection of D-GaIN and LPS was observed and the survival rates were calculated.The mice were sacrificed and livers were removed for microscopic examination of pathologic changes.Results Compared with group Ⅰ,the activities of AST and ALT were significantly increased at each time point in Ⅱ and Ⅲ groups and the serum TNF-α concentrations at T1,2 and IL-1β and IL-10 concentrations at each time point were significantly increased in group Ⅱ,and the serum TNF-α concentrations at T1,and IL-1β and IL-10 concentrations at T2,3 were significantly increased in group Ⅲ (P ＜ 0.05).Compared with group Ⅱ,the activities of AST and ALT at each time point,serum TNF-α concentrations at T1,2 and IL-1β and IL-10 concentrations at T2,3 were significantly decreased and the survival rate within 12 h after injection of D-GaIN and LPS was increased in group Ⅲll (P ＜ 0.05).The pathologic changes of liver tissues were gradually attenuated in Ⅱ and Ⅲ groups.Conclusion Propofol can reduce the liver injury in mice with ALF through inhibiting inflammatory responses.