中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
8期
24-26
,共3页
李军%郝彩琴%马小兰%王海霞%王建建%冷晓红
李軍%郝綵琴%馬小蘭%王海霞%王建建%冷曉紅
리군%학채금%마소란%왕해하%왕건건%랭효홍
苦豆籽%总生物碱%正交试验%超声提取
苦豆籽%總生物堿%正交試驗%超聲提取
고두자%총생물감%정교시험%초성제취
Sophora Alopecuroides L.%Total alkaloids%Orthogonal design%Ultrasonic extraction
目的：优选苦豆子中总生物碱的超声提取工艺。方法：以总生物碱提取率为指标，采用单因素和正交试验考察料液比、超声温度、乙醇浓度、超声时间、提取次数对提取工艺的影响，确定最佳提取工艺。结果：各因素对超声提取工艺的影响顺序为超声温度＞料液比＞超声时间＞乙醇浓度；最佳提取工艺为用65％乙醇，按料液比1：16，温度30℃，超声提取2次，每次20min。结论：优选的提取工艺稳定可行，提取率高。
目的：優選苦豆子中總生物堿的超聲提取工藝。方法：以總生物堿提取率為指標，採用單因素和正交試驗攷察料液比、超聲溫度、乙醇濃度、超聲時間、提取次數對提取工藝的影響，確定最佳提取工藝。結果：各因素對超聲提取工藝的影響順序為超聲溫度＞料液比＞超聲時間＞乙醇濃度；最佳提取工藝為用65％乙醇，按料液比1：16，溫度30℃，超聲提取2次，每次20min。結論：優選的提取工藝穩定可行，提取率高。
목적：우선고두자중총생물감적초성제취공예。방법：이총생물감제취솔위지표，채용단인소화정교시험고찰료액비、초성온도、을순농도、초성시간、제취차수대제취공예적영향，학정최가제취공예。결과：각인소대초성제취공예적영향순서위초성온도＞료액비＞초성시간＞을순농도；최가제취공예위용65％을순，안료액비1：16，온도30℃，초성제취2차，매차20min。결론：우선적제취공예은정가행，제취솔고。
Objective To optimize ultrasonic extraction process of total alkaloids from Sophora Alopecuroides L..Method With extraction rate of total alkaloids as index,effects of liquid -solid ratio,ultrasonic temperature,ethanol concentration,ultrasonic time,extraction times on extraction process were investigated by single factor test and orthogonal test.Result The influencing order of each factors on ultrasonic extraction process of total alkaloids was ultrasonic temperature>liquid-solid ratio>extraction time>ethanol concentration.Optimum extraction process was as following:ultrasonic extracted 2 times with 16 times the amount of 65%ethanol,20 min one time,ultrasonic temperature 30℃.Conclusions This optimized process was stable and feasible with high extraction rate.
