中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
11期
856-858
,共3页
壬基酚%胰岛素样因子-3%大鼠%睾丸
壬基酚%胰島素樣因子-3%大鼠%睪汍
임기분%이도소양인자-3%대서%고환
Nonylphenol%Insulin like 3 gene%Rots%Testis
目的 探讨母体染毒壬基酚(NP)对雄性胎鼠生殖发育的影响,并检测胎鼠睾丸组织中胰岛素样因子-3(Insl-3)的mRNA以及蛋白的表达.方法 将已受孕的SD雌性大鼠随机分为对照组、5、40、100、200 mg/kg NP染毒组(每组20只),染毒组大鼠于孕14～19 d以NP灌胃染毒,对照组大鼠用纯花生油灌胃第19天处死孕鼠后检测孕鼠和雄性胎鼠生殖发育相关指标；反转录-聚合酶链式反应(RT-PCR)和蛋白免疫印迹技术(Western blot)检测其睾丸组织中Insl-3的mRNA及蛋白的表达.结果 与对照组比较,40、100、200 mg/kg组胎鼠体重和睾丸系数明显降低,差异有统计学意义(P＜0.05或P＜0.01).与对照组比较,40、100、200 mg/kg组的睾丸下降程度降低,差异有统计学意义(P＜0.05).与对照组比较,40、100、200 mg/kg组Insl-3mRNA及蛋白表达降低,差异有统计学意义(P＜0.05或P＜0.01).结论 孕期暴露NP可致其雄性胎鼠睾丸发育不良,下降不全,并且可下调Insl-3的表达.
目的 探討母體染毒壬基酚(NP)對雄性胎鼠生殖髮育的影響,併檢測胎鼠睪汍組織中胰島素樣因子-3(Insl-3)的mRNA以及蛋白的錶達.方法 將已受孕的SD雌性大鼠隨機分為對照組、5、40、100、200 mg/kg NP染毒組(每組20隻),染毒組大鼠于孕14～19 d以NP灌胃染毒,對照組大鼠用純花生油灌胃第19天處死孕鼠後檢測孕鼠和雄性胎鼠生殖髮育相關指標；反轉錄-聚閤酶鏈式反應(RT-PCR)和蛋白免疫印跡技術(Western blot)檢測其睪汍組織中Insl-3的mRNA及蛋白的錶達.結果 與對照組比較,40、100、200 mg/kg組胎鼠體重和睪汍繫數明顯降低,差異有統計學意義(P＜0.05或P＜0.01).與對照組比較,40、100、200 mg/kg組的睪汍下降程度降低,差異有統計學意義(P＜0.05).與對照組比較,40、100、200 mg/kg組Insl-3mRNA及蛋白錶達降低,差異有統計學意義(P＜0.05或P＜0.01).結論 孕期暴露NP可緻其雄性胎鼠睪汍髮育不良,下降不全,併且可下調Insl-3的錶達.
목적 탐토모체염독임기분(NP)대웅성태서생식발육적영향,병검측태서고환조직중이도소양인자-3(Insl-3)적mRNA이급단백적표체.방법 장이수잉적SD자성대서수궤분위대조조、5、40、100、200 mg/kg NP염독조(매조20지),염독조대서우잉14～19 d이NP관위염독,대조조대서용순화생유관위제19천처사잉서후검측잉서화웅성태서생식발육상관지표；반전록-취합매련식반응(RT-PCR)화단백면역인적기술(Western blot)검측기고환조직중Insl-3적mRNA급단백적표체.결과 여대조조비교,40、100、200 mg/kg조태서체중화고환계수명현강저,차이유통계학의의(P＜0.05혹P＜0.01).여대조조비교,40、100、200 mg/kg조적고환하강정도강저,차이유통계학의의(P＜0.05).여대조조비교,40、100、200 mg/kg조Insl-3mRNA급단백표체강저,차이유통계학의의(P＜0.05혹P＜0.01).결론 잉기폭로NP가치기웅성태서고환발육불량,하강불전,병차가하조Insl-3적표체.
Objective To investigate the effect of nonylphenol (NP) exposure on the genital development of fetal male rats in pregnant rats,and to measure the mRNA and protein expression of insulin-like factor 3 (Insl-3) in the testicular tissue of fetal rats.Methods A total of 100 pregnant SD rats were equally assigned to blank control group and four NP treated groups.Each rat in the NP treated groups received intragastric administration of NP at doses of 5,40,100,or 200 mg/kg/d from day 14 to 19 of gestation,and the rats in the blank control group received intragastric administration of pure peanut oil.The pregnant rats were sacrificed on day 19 of gestation.The body weight and testicular weight of each fetal rat were measured,and the descent of testis was also observed.The mRNA and protein expression of Insl-3 in the testicular tissue of fetal rats was analyzed by reverse transcription-PCR and Western blot.Results Compared with the blank control group,the 40,100,and 200 mg/kg NP treated groups showed significantly decreased body weight and weight coefficient of testis (P＜0.05 or P＜0.01),significantly decreased testicular descent (P＜0.05),and significantly decreased mRNA and protein expression of Insl-3 (P＜0.05 or P＜0.01).Conclusion Exposure to nonylphenol can lead to testicular maldevelopment,incomplete testicular descent,and Insl-3 expression downregulation of fetal male rats in pregnant rats.
