CAJ | 학술논문

目的研究血清中神经生长因子(nerve growth factor,NGF)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、白细胞介素6(interleukin 6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、干扰素-γ(interferon gamma,IFN-γ)、钙结合蛋白(calcium binding protein,S100β)、髓鞘碱性蛋白(myelin basic protein,MBP)和神经胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)8种蛋白因子水平对精神分裂症的诊断价值.方法用酶联免疫吸附技术(enzyme linked immunosorbent assay,ELISA)测定106例精神分裂症患者、105例抑郁症患者和120例正常对照者血清8种蛋白因子的浓度.用受试者工作特征(receiver operating characteristic,ROC)曲线评估8个因子的诊断价值.结果精神分裂症患者组血清NGF浓度低于抑郁症组和正常对照组[(32.55±2.12)ng/L vs (38.90±10.47)ng/L、(40.54±6.71)ng/L],差异有统计学意义(P<0.01).精神分裂者组血清NGF诊断截断值35.73ng/L对应最佳灵敏度为86.7%,特异度为92.5%(95%CI=0.68~0.95),抑郁症组血清NGF诊断截断值36.04ng/L对应最佳灵敏度为85.8%,特异度为49.5%(95%CI=0.50~0.66),ROC曲线下面积(areas under the ROC curve,AUC)分别为0.91、0.58.采用逐步判别分析(stepwise discriminant analysis,SDA)进行数据的重新归类,从中发现NGF、IL-6、S100β、MBP和GFAP 5个因子浓度对协助诊断精神分裂症的贡献率更大,联合用5个因子的诊断价值均高于任何单种蛋白因子的诊断价值.结论应用蛋白因子浓度水平诊断精神分裂症是可行的,并有望进一步对精神分裂症的客观实验室检查提供新思路.
목적 연구혈청중신경생장인자(nerve growth factor,NGF)、뇌원성신경영양인자(brain-derived neurotrophic factor,BDNF)、백세포개소6(interleukin 6,IL-6)、종류배사인자-α(tumor necrosis factor alpha,TNF-α)、간우소-γ(interferon gamma,IFN-γ)、개결합단백(calcium binding protein,S100β)、수초감성단백(myelin basic protein,MBP)화신경효질섬유산성단백(glial fibrillary acidic protein,GFAP)8충단백인자수평대정신분렬증적진단개치.방법 용매련면역흡부기술(enzyme linked immunosorbent assay,ELISA)측정106례정신분렬증환자、105례억욱증환자화120례정상대조자혈청8충단백인자적농도.용수시자공작특정(receiver operating characteristic,ROC)곡선평고8개인자적진단개치.결과 정신분렬증환자조혈청NGF농도저우억욱증조화정상대조조[(32.55±2.12)ng/L vs (38.90±10.47)ng/L、(40.54±6.71)ng/L],차이유통계학의의(P<0.01).정신분렬자조혈청NGF진단절단치35.73ng/L대응최가령민도위86.7%,특이도위92.5%(95%CI=0.68~0.95),억욱증조혈청NGF진단절단치36.04ng/L대응최가령민도위85.8%,특이도위49.5%(95%CI=0.50~0.66),ROC곡선하면적(areas under the ROC curve,AUC)분별위0.91、0.58.채용축보판별분석(stepwise discriminant analysis,SDA)진행수거적중신귀류,종중발현NGF、IL-6、S100β、MBP화GFAP 5개인자농도대협조진단정신분렬증적공헌솔경대,연합용5개인자적진단개치균고우임하단충단백인자적진단개치.결론 응용단백인자농도수평진단정신분렬증시가행적,병유망진일보대정신분렬증적객관실험실검사제공신사로.