国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
2期
135-137
,共3页
金丝桃苷%治带片%高效液相色谱法
金絲桃苷%治帶片%高效液相色譜法
금사도감%치대편%고효액상색보법
Hyperoside%Zhidai tablet%HPLC
目的 建立测定治带片中金丝桃苷含量的高效液相色谱方法.方法 采用PhenomenexLUNA-C 18柱(4.6 mm×250 mm,5 μm),以乙腈:0.1％磷酸溶液(10∶90)为流动相,流速1.0 ml/min,柱温30℃,检测波长360 nm.结果 金丝桃苷在1.2～24.0 μg/ml浓度范围内与峰面积呈良好线性关系(r=0.9998),平均加样回收率为98.4％,RSD 0.93％.结论 本方法简便,快速,准确,重复性好,可用于治带片中金丝桃苷的含量测定.
目的 建立測定治帶片中金絲桃苷含量的高效液相色譜方法.方法 採用PhenomenexLUNA-C 18柱(4.6 mm×250 mm,5 μm),以乙腈:0.1％燐痠溶液(10∶90)為流動相,流速1.0 ml/min,柱溫30℃,檢測波長360 nm.結果 金絲桃苷在1.2～24.0 μg/ml濃度範圍內與峰麵積呈良好線性關繫(r=0.9998),平均加樣迴收率為98.4％,RSD 0.93％.結論 本方法簡便,快速,準確,重複性好,可用于治帶片中金絲桃苷的含量測定.
목적 건립측정치대편중금사도감함량적고효액상색보방법.방법 채용PhenomenexLUNA-C 18주(4.6 mm×250 mm,5 μm),이을정:0.1％린산용액(10∶90)위류동상,류속1.0 ml/min,주온30℃,검측파장360 nm.결과 금사도감재1.2～24.0 μg/ml농도범위내여봉면적정량호선성관계(r=0.9998),평균가양회수솔위98.4％,RSD 0.93％.결론 본방법간편,쾌속,준학,중복성호,가용우치대편중금사도감적함량측정.
Objective To establish a HPLC method for the determination of hyperoside in Zhidai tablet.Method The HPLC system used the Phenomenex LUNA-C18 (4.6 mm× 250 mm,5 μm)column,the mobile phase consisted of acetonitrile (10)-0.1％ H3PO4 (90),the flow rate was 1.0 ml/min,the column temperature was set at 30℃,the detection wavelength was set at 360 nm.Results The linear response range was 1.2～24.0 μg/mL(r=0.9998).The average recovery of mangiferin was 98.4％,and RSD was 0.93％.Conclusions The method is simple,rapid,accurate and repeatable.It can be applied in the determination of hyperoside in Zhidai tablet.
