分别利用浓度为10、50、100、150 μmol/L的CdCl2处理萝卜幼苗,分析根系全蛋白质组和根系线粒体蛋白质组发生的变化,并对其中100 μmol/L的CdCl2诱导下萝卜根系的差异表达蛋白质组作详细分析,利用质谱技术鉴别差异蛋白的归属.根系全蛋白中有22个蛋白质斑点显示出差异表达,其中11个斑点(斑点4、斑点9～18)下调,4个斑点(斑点19～22)上调,7个斑点(斑点1～3,5～8)被抑制表达.线粒体中有22个蛋白质斑点显示差异表达,其中19个斑点(斑点1～19)下调,2个斑点(斑点20～21)上调,斑点22被诱导合成,经CdCl2诱导后,萝卜根系内的蛋白质组产生明显的差异表达现象,这些蛋白质分别是:cruciferin,maturase K,DNA-binding protein,defence-related protein,F-box family protein,F-actin capping protein beta subunit,transcription factor,monodehydroascorbate reductase,chaperone binding protein,SHLI,RNA polymerase beta chain,ATF3,putative acyl transferase 4,transposon protein putative mutator sub-class,transposon protein putative En/spm sub-class,retransposon protein,putative Tyl-copia sub-class,isocitrate lyase,phytochrome kinase substrate-related,phosphoenolpyruvate carboxylase,serine/threonine-protein kinase,ATP synthase beta chain,ARol-like protein 4,catalase isozyme 1,pentatricopeptide (PPR)repeat-countaining protein,cytochrome p450,DNA polymerase Ⅰ,trihelix transcription factor,NADH dehydrogenase submit F,cycline-dependent kinase,F-box protein-related,ATP synthase subunit beta,mitochondrial precursor,glucan phosphorylase putative,zinc finger family protein,heat shock protein 26,peroxiredoxin type2 putative,alcohol dehydrogenase.这些差异表达的蛋白质,其功能涉及DNA、RNA及蛋白质合成,基因转录、转座与内含子剪接,细胞信号转导与结构维持,以及不良环境条件下的防御与能量代谢等方面.
分彆利用濃度為10、50、100、150 μmol/L的CdCl2處理蘿蔔幼苗,分析根繫全蛋白質組和根繫線粒體蛋白質組髮生的變化,併對其中100 μmol/L的CdCl2誘導下蘿蔔根繫的差異錶達蛋白質組作詳細分析,利用質譜技術鑒彆差異蛋白的歸屬.根繫全蛋白中有22箇蛋白質斑點顯示齣差異錶達,其中11箇斑點(斑點4、斑點9～18)下調,4箇斑點(斑點19～22)上調,7箇斑點(斑點1～3,5～8)被抑製錶達.線粒體中有22箇蛋白質斑點顯示差異錶達,其中19箇斑點(斑點1～19)下調,2箇斑點(斑點20～21)上調,斑點22被誘導閤成,經CdCl2誘導後,蘿蔔根繫內的蛋白質組產生明顯的差異錶達現象,這些蛋白質分彆是:cruciferin,maturase K,DNA-binding protein,defence-related protein,F-box family protein,F-actin capping protein beta subunit,transcription factor,monodehydroascorbate reductase,chaperone binding protein,SHLI,RNA polymerase beta chain,ATF3,putative acyl transferase 4,transposon protein putative mutator sub-class,transposon protein putative En/spm sub-class,retransposon protein,putative Tyl-copia sub-class,isocitrate lyase,phytochrome kinase substrate-related,phosphoenolpyruvate carboxylase,serine/threonine-protein kinase,ATP synthase beta chain,ARol-like protein 4,catalase isozyme 1,pentatricopeptide (PPR)repeat-countaining protein,cytochrome p450,DNA polymerase Ⅰ,trihelix transcription factor,NADH dehydrogenase submit F,cycline-dependent kinase,F-box protein-related,ATP synthase subunit beta,mitochondrial precursor,glucan phosphorylase putative,zinc finger family protein,heat shock protein 26,peroxiredoxin type2 putative,alcohol dehydrogenase.這些差異錶達的蛋白質,其功能涉及DNA、RNA及蛋白質閤成,基因轉錄、轉座與內含子剪接,細胞信號轉導與結構維持,以及不良環境條件下的防禦與能量代謝等方麵.
분별이용농도위10、50、100、150 μmol/L적CdCl2처리라복유묘,분석근계전단백질조화근계선립체단백질조발생적변화,병대기중100 μmol/L적CdCl2유도하라복근계적차이표체단백질조작상세분석,이용질보기술감별차이단백적귀속.근계전단백중유22개단백질반점현시출차이표체,기중11개반점(반점4、반점9～18)하조,4개반점(반점19～22)상조,7개반점(반점1～3,5～8)피억제표체.선립체중유22개단백질반점현시차이표체,기중19개반점(반점1～19)하조,2개반점(반점20～21)상조,반점22피유도합성,경CdCl2유도후,라복근계내적단백질조산생명현적차이표체현상,저사단백질분별시:cruciferin,maturase K,DNA-binding protein,defence-related protein,F-box family protein,F-actin capping protein beta subunit,transcription factor,monodehydroascorbate reductase,chaperone binding protein,SHLI,RNA polymerase beta chain,ATF3,putative acyl transferase 4,transposon protein putative mutator sub-class,transposon protein putative En/spm sub-class,retransposon protein,putative Tyl-copia sub-class,isocitrate lyase,phytochrome kinase substrate-related,phosphoenolpyruvate carboxylase,serine/threonine-protein kinase,ATP synthase beta chain,ARol-like protein 4,catalase isozyme 1,pentatricopeptide (PPR)repeat-countaining protein,cytochrome p450,DNA polymerase Ⅰ,trihelix transcription factor,NADH dehydrogenase submit F,cycline-dependent kinase,F-box protein-related,ATP synthase subunit beta,mitochondrial precursor,glucan phosphorylase putative,zinc finger family protein,heat shock protein 26,peroxiredoxin type2 putative,alcohol dehydrogenase.저사차이표체적단백질,기공능섭급DNA、RNA급단백질합성,기인전록、전좌여내함자전접,세포신호전도여결구유지,이급불량배경조건하적방어여능량대사등방면.
