中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
3期
387-389
,共3页
肝炎病毒,乙型%丝裂原激活蛋白激酶%信号转导
肝炎病毒,乙型%絲裂原激活蛋白激酶%信號轉導
간염병독,을형%사렬원격활단백격매%신호전도
Hepatitis B virus%Mitogen activator protien kinase%Signal transduction
目的 通过观察孕妇胎盘组织的丝裂原激活蛋白激酶(MAPK)表达与乙型肝炎病毒(HBV)持续感染的关系,探讨细胞内信号变化与HBV宫内传播分子机制.方法 研究对象为2010年1月至2011年11月在我院自然分娩或剖宫产的慢性乙型肝炎产妇52例,分为HBeAg阳性组(22例)和HBeAg阴性组(25例),5例肝功能正常、HBV和丙型肝炎病毒(HCV)血清标志物均为阴性的产妇为正常对照组.均于分娩后迅速无菌收集胎盘组织,应用定量逆转录聚合酶链反应(RT-PCR)方法检测胎盘组织中MAPKmRNA水平,并分别定量检测其血清HBV-DNA和HBeAg水平.结果 HBeAg阳性组HBV-DNA水平高于HBeAg阴性组,组间差异有统计学意义[(0.6±0.5)×106拷贝/L比(4.6±2.1)x1010拷贝/L,P＜0.01].正常对照组、HBeAg阴性组和阳性组的MAPK基因表达分别为0.98、0.70、0.56,3组之间分别两两比较,差异均有统计学意义(均P＜0.05).结论 胎盘组织中MAPK的表达与其持续感染HBV有一定关系.
目的 通過觀察孕婦胎盤組織的絲裂原激活蛋白激酶(MAPK)錶達與乙型肝炎病毒(HBV)持續感染的關繫,探討細胞內信號變化與HBV宮內傳播分子機製.方法 研究對象為2010年1月至2011年11月在我院自然分娩或剖宮產的慢性乙型肝炎產婦52例,分為HBeAg暘性組(22例)和HBeAg陰性組(25例),5例肝功能正常、HBV和丙型肝炎病毒(HCV)血清標誌物均為陰性的產婦為正常對照組.均于分娩後迅速無菌收集胎盤組織,應用定量逆轉錄聚閤酶鏈反應(RT-PCR)方法檢測胎盤組織中MAPKmRNA水平,併分彆定量檢測其血清HBV-DNA和HBeAg水平.結果 HBeAg暘性組HBV-DNA水平高于HBeAg陰性組,組間差異有統計學意義[(0.6±0.5)×106拷貝/L比(4.6±2.1)x1010拷貝/L,P＜0.01].正常對照組、HBeAg陰性組和暘性組的MAPK基因錶達分彆為0.98、0.70、0.56,3組之間分彆兩兩比較,差異均有統計學意義(均P＜0.05).結論 胎盤組織中MAPK的錶達與其持續感染HBV有一定關繫.
목적 통과관찰잉부태반조직적사렬원격활단백격매(MAPK)표체여을형간염병독(HBV)지속감염적관계,탐토세포내신호변화여HBV궁내전파분자궤제.방법 연구대상위2010년1월지2011년11월재아원자연분면혹부궁산적만성을형간염산부52례,분위HBeAg양성조(22례)화HBeAg음성조(25례),5례간공능정상、HBV화병형간염병독(HCV)혈청표지물균위음성적산부위정상대조조.균우분면후신속무균수집태반조직,응용정량역전록취합매련반응(RT-PCR)방법검측태반조직중MAPKmRNA수평,병분별정량검측기혈청HBV-DNA화HBeAg수평.결과 HBeAg양성조HBV-DNA수평고우HBeAg음성조,조간차이유통계학의의[(0.6±0.5)×106고패/L비(4.6±2.1)x1010고패/L,P＜0.01].정상대조조、HBeAg음성조화양성조적MAPK기인표체분별위0.98、0.70、0.56,3조지간분별량량비교,차이균유통계학의의(균P＜0.05).결론 태반조직중MAPK적표체여기지속감염HBV유일정관계.
Objective To study molecule mechanism of signal transduction signal transduction signal transduction; to observe correlation between the intrauterine transmission of hepatitis B virus (HBV) and expression of mitogen activator protien kinase (MAPK) in placenta of chronicity type b hepatitis pregnant women.Methods There were 47 chronicity type B hepatitis pregnant women who were divided in active hepatitis B e antigens(HBeAg) group (22 cases) and negative HBeAg group(25 cases).Blank group(5 cases) included normal pregnant women.The expression of MAPK mRNA in placenta tissue was detected through the method of reverse transcription-polymerase chain reaction (RT-PCR).The level of HBV-DNA and HBeAg in blood serum was also detected.Results The level of HBV-DNA in blank group,negative HBeAg group and active HBeAg group increased (P ＜0.01).The expression of MAPK mRNA in placenta tissue in blank group,negative HBeAg group and active HBeAg group were 0.98,0.70 and 0.56,and there was significant difference among three groups increased (P ＜ 0.05).Conclusion There is a certain relation between the intrauterine transmission of HBV and expression of MAPK mRNA in placenta tissue which can be influenced by the level of HBV-DNA in blood serum with down regulation.
