CAJ | 학술논문

以羊软骨为试验材料，对前处理工艺-脱脂、脱钙工艺进行了改进。经过单因素实验筛选确定了较为理想的脱脂剂为氯仿：甲醇：水=1：2：0．8，脱脂条件是温度为25℃、时间为48h、固液比1：10时其脱脂率为最高，达55．88％；选择0．5mol／LEDTA为脱钙试剂时脱钙率最高，达29．16％；SDS—PAGE电泳结果表明：用前处理改进后提取的的胶原蛋白与sigma公司的Ⅱ型标准品相一致，证明羊软骨中存在的蛋白主要是Ⅱ型胶原蛋白。紫外光谱分析结果显示：所提胶原的最大吸收峰为230nm左右，与Ⅱ型标准品最大相一致。
이양연골위시험재료，대전처리공예-탈지、탈개공예진행료개진。경과단인소실험사선학정료교위이상적탈지제위록방：갑순：수=1：2：0．8，탈지조건시온도위25℃、시간위48h、고액비1：10시기탈지솔위최고，체55．88％；선택0．5mol／LEDTA위탈개시제시탈개솔최고，체29．16％；SDS—PAGE전영결과표명：용전처리개진후제취적적효원단백여sigma공사적Ⅱ형표준품상일치，증명양연골중존재적단백주요시Ⅱ형효원단백。자외광보분석결과현시：소제효원적최대흡수봉위230nm좌우，여Ⅱ형표준품최대상일치。
The sheep cartilages were collected as raw materials, Optimization of degreasation and decalcification process during ex- tracting collagen from sheep cartilages were investigated. Improved process of degreasing and decalcification were confinued . The opti- mum parameters of degreasing were : temperature 25~C , degreasing time 48h, chloroform : methanol : water = 1 : 2:0.8. When ratio of Solvent to Solid was 1 : 10 ,the ratio of degreasing was 55.88%. The optimum decalcifying agent was 0.5mol/L EDTA and the ratio of decalcification was 29.16 %. The extracted collagen with improved process was same with the protein and Sigma C - II by SDS - PAGE. The absorption peak of C - II in UV was about 230 nm. The results showed that the collagen type II was mainly protein in sheep cartilage.