CAJ | 학술논문

目的评价脊髓MCP/DAF表达在大鼠神经病理性痛形成中的作用.方法 MCP/DAF双基因重组转染SD大鼠24只,体重200 ～ 250 g,按照随机数字表法分为2组(n=12):转染大鼠假手术组(Rsham组)和转染大鼠坐骨神经慢性压迫损伤组(RCCI组);健康雄性SD大鼠24只,体重200～250 g,按照随机数字表法分为2组(n=12):正常大鼠假手术组(Nsham组)和正常大鼠坐骨神经慢性压迫性损伤组(NCCI组).RCCI组和NCCI组大鼠行右侧坐骨神经四道环形结扎,Rsham组和Nsham组大鼠只暴露右侧坐骨神经,不予环形结扎.分别于术前1d和术后1、3和7d时测定大鼠的热痛阈和机械痛阈.术后7d时测定痛阈后处死大鼠,取k,5脊髓,采用免疫组化法检测0X-42的表达,RTPCR法检测MCP mRNA和DAF mRNA的表达.结果与Nsham组比较,NCCI组大鼠术后1、3和7d时热痛阈和机械痛阈降低,脊髓OX-42表达上调,MCP mRNA和DAF mRNA表达下调(P＜0.05),Rsham组和RCCI组大鼠术后上述时点热痛阈、机械痛阈和脊髓OX-42表达差异无统计学意义(P＞0.05),MCP mRNA和DAF mRNA表达上调(P＜0.05);与NCCI组比较,RCCI组大鼠术后上述时点热痛阈和机械痛阈升高,脊髓OX-42表达下调,MCP mRNA和DAF mRNA表达上调(p＜0.05).结论 MCP/DAF表达上调抑制大鼠神经病理性痛的形成,其机制与调节脊髓小胶质细胞活化有关.
목적 평개척수MCP/DAF표체재대서신경병이성통형성중적작용.방법 MCP/DAF쌍기인중조전염SD대서24지,체중200 ～ 250 g,안조수궤수자표법분위2조(n=12):전염대서가수술조(Rsham조)화전염대서좌골신경만성압박손상조(RCCI조);건강웅성SD대서24지,체중200～250 g,안조수궤수자표법분위2조(n=12):정상대서가수술조(Nsham조)화정상대서좌골신경만성압박성손상조(NCCI조).RCCI조화NCCI조대서행우측좌골신경사도배형결찰,Rsham조화Nsham조대서지폭로우측좌골신경,불여배형결찰.분별우술전1d화술후1、3화7d시측정대서적열통역화궤계통역.술후7d시측정통역후처사대서,취k,5척수,채용면역조화법검측0X-42적표체,RTPCR법검측MCP mRNA화DAF mRNA적표체.결과 여Nsham조비교,NCCI조대서술후1、3화7d시열통역화궤계통역강저,척수OX-42표체상조,MCP mRNA화DAF mRNA표체하조(P＜0.05),Rsham조화RCCI조대서술후상술시점열통역、궤계통역화척수OX-42표체차이무통계학의의(P＞0.05),MCP mRNA화DAF mRNA표체상조(P＜0.05);여NCCI조비교,RCCI조대서술후상술시점열통역화궤계통역승고,척수OX-42표체하조,MCP mRNA화DAF mRNA표체상조(p＜0.05).결론 MCP/DAF표체상조억제대서신경병이성통적형성,기궤제여조절척수소효질세포활화유관.
Objective To evaluate the role of MCP/DAF expression in the spinal cord in the development of neuropathic pain (NP) induced by chronic constrictive injury (CCI) of the sciatic nerve in rats.Methods Twenty-four Sprague-Dawley rats transfected with MCP/DAF,weighing 200-250 g,were randomly divided into 2 groups (n =12 each) using a random number table:sham operation of transfected rat group (Rsham group) and CCI of transfected rat group (RCCI group).Twenty-four healthy male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 2 groups (n =12 each) using a random number table:sham operation of normal rat group (Nsham group) and CCI of normal rat group (NCCI group).The right sciatic nerve was exposed and 4 loose ligatures wen placed on the sciatic nerve at 1 mm intervals with 4-0 catgut in RCCI and NCCI groups.The right sciatic nerve was only exposed in Rsham and Nsham groups.Paw withdrawal threshold to yon Frey filament stimulation (PWT) and paw withdrawal latency to nociceptive thermal stimulation (PWL) were measured at 1 day before operation (baseline) and 1,3 and 7 days after operation.The animals were sacrificed after measurement of pain threshold on 7 days after operation and the L4,5 segment of the spinal cord was removed for determination of the expression of OX-42 (by immuno-histochemistry) and MCP mRNA and DAF mRNA (by RT-PCR).Results Compared with Nsham group,the PWT and PWL were significantly decreased on 1,3 and 7 days after operation,the expression of OX-42 was up-regulated,and the expression of MCP mRNA and DAF mRNA was down-regulated in NCCI group (P ＜ 0.05),and no significant changes were found in the PWT and PWL on 1,3 and 7 days after operation and expression of OX-42(P ＞ 0.05),and the expression of MCP mRNA and DAF mRNA was up-regulated in Rsham and RCCI groups (P ＞ 0.05).Compared with NCCI group,the PWT and PWL were significantly increased on 1,3 and 7 days after operation,the expression of OX-42 was down-regulated,and the expression of MCP mRNA and DAF mRNA was up-regulated in RCCI group (P ＜ 0.05).Conclusion Up-regulation of MCP/ DAF expression in the spinal cord can inhibit the development of NP in rats and regulation of activation of microglias in the spinal cord is involved in the mechanism.