CAJ | 학술논문

目的拟制定适合本实验室血细胞仪器分析后血涂片复审准则,以保证血细胞分析结果的准确性和可靠性,更有利于临床对疾病的诊断和治疗.方法在雅培CELL-DYN3700五分类血细胞计数仪上检测1486份静脉血,先以“国际血涂片复审准则”为标准,记录仪器检测所得各项数据、报警类别和显微镜下所见.结合本实验室实际情况和仪器性能特点,制定适合的血涂片复审准则,并以200份标本验证.结果以“41条规则”复审,1486份标本真阴性60.4％、真阳性20.8％、假阴性3.0％、假阳性15.8％.以本实验室血涂片复审准则验证200份标本,真阴性69.0％、真阳性18.5％、假阴性2.5％、假阳性10.0％,具有诊断意义的重要参数(幼稚细胞)无漏检.复检率由于36.6％下降到达28.5％.结论血细胞仪器分析具有局限性,对于形态结构异常的细胞不能作出准确的判断和定位,实验室应根据自身实际情况制定合适的血涂片复审准则,以保证血细胞分析结果的准确性和可靠性.
목적 의제정괄합본실험실혈세포의기분석후혈도편복심준칙,이보증혈세포분석결과적준학성화가고성,경유리우림상대질병적진단화치료.방법 재아배CELL-DYN3700오분류혈세포계수의상검측1486빈정맥혈,선이“국제혈도편복심준칙”위표준,기록의기검측소득각항수거、보경유별화현미경하소견.결합본실험실실제정황화의기성능특점,제정괄합적혈도편복심준칙,병이200빈표본험증.결과 이“41조규칙”복심,1486빈표본진음성60.4％、진양성20.8％、가음성3.0％、가양성15.8％.이본실험실혈도편복심준칙험증200빈표본,진음성69.0％、진양성18.5％、가음성2.5％、가양성10.0％,구유진단의의적중요삼수(유치세포)무루검.복검솔유우36.6％하강도체28.5％.결론 혈세포의기분석구유국한성,대우형태결구이상적세포불능작출준학적판단화정위,실험실응근거자신실제정황제정합괄적혈도편복심준칙,이보증혈세포분석결과적준학성화가고성.
Objective To ensure the accuracy and reliability of the results of blood cell analysis and help clinical diagnosis and treatment making reexamination criteria for blood smear analyzed by blood cell analyzer in our laboratory.Methods 1486 blood samples were detected by Abbott CELL-DYN3700 hematology analyzer.The data detected by the equipment,the alarm-giving class,and microscopic findings were recorded according to the "international criteria for blood smear reexamination".Appropriate criteria for blood smear reexamination were established in terms of the actual situation and performance characteristics of the instruments in our laboratory.The criteria were verified using 200 specimens.Results The reexamination according to "41 rules" showed that the percentages of true negative,true positive,false negative,and false positive results of the 1486 specimens were 60.4％,20.8％,3.0％,and 15.8％,respectively and were 69.0％,18.5％,2.5％,and 10.0％,respectively after being verified using 200 specimens.The important parameter (immature cells) with diagnostic significance hadn' t been missed.The reexamination rate dropped from 36.6％ to 28.5％.Conclusions Blood cell analyzer can' t make accurate judgment and location for cells with abnormal morphology.Therefore,the laboratory should establish some appropriate reexamination criteria for blood smear according to actual situation to ensure the accuracy and reliability of the results of blood cell analysis.