CAJ | 학술논문

目的：了解瘦素对肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导肝星状细胞（HSC）凋亡的影响及调控机制。方法MTT比色法、流式细胞术检测瘦素对外源性TRAIL诱导HSC-T6细胞增殖和细胞凋亡的影响；采用Western印迹检测信号传导与转录激活因子3（STAT3）、磷酸化STAT3（pSTAT3）、Bcl-2。结果TRAIL抑制HSC-T6细胞增殖、诱导HSC-T6细胞凋亡，而瘦素使TRAIL诱导的HSC-T6细胞凋亡明显减少（P＜0．05）；当TRAIL诱导HSC-T6细胞凋亡时加入瘦素，pSTAT3、Bcl-2表达上调。结论瘦素可以使外源性TRAIL诱导的HSC-T6细胞凋亡减少，可能与瘦素促使STAT3磷酸化，Bcl-2表达上调有关。
목적：료해수소대종류배사인자상관조망유도배체（TRAIL）유도간성상세포（HSC）조망적영향급조공궤제。방법MTT비색법、류식세포술검측수소대외원성TRAIL유도HSC-T6세포증식화세포조망적영향；채용Western인적검측신호전도여전록격활인자3（STAT3）、린산화STAT3（pSTAT3）、Bcl-2。결과TRAIL억제HSC-T6세포증식、유도HSC-T6세포조망，이수소사TRAIL유도적HSC-T6세포조망명현감소（P＜0．05）；당TRAIL유도HSC-T6세포조망시가입수소，pSTAT3、Bcl-2표체상조。결론수소가이사외원성TRAIL유도적HSC-T6세포조망감소，가능여수소촉사STAT3린산화，Bcl-2표체상조유관。
Objective To determine whether leptin protects rat hepatic stellate cells (HSC) HSC-T6 from TRAIL-induced apoptosis and its pathogenesis .Methods HSC-T6 cells were cultured and treated with TRAIL and leptin .The inhibition on proliferation of HSC-T6 cells was detected by 3-(4 ,5-dimethytthiazole-2-yl)-2 ,5-diphenyltetrazolium bromide (MTT) assay ,while apoptosis rate was detected by flow Cytometry .Western blot was performed to determine STAT3 ,pSTAT3 and Bcl-2 in HSC-T6 cells .Results Leptin increased cell proliferation of HSC-T6 cells treated with TRAIL and reduced apoptosis rate (P< 0 .05) .Leptin pSTAT3 and Bcl-2 expression was increased by leptin using Western blot .Conclusion Leptin could reduce apoptosis of HSC-T6 cells treated with TRAIL effectively ,which was associated with high expressions of pSTAT 3 and Bcl-2 .