CAJ | 학술논문

目的:探讨预防性给予重组人红细胞生成素对雄性大鼠L5脊神经切断大鼠机械、热痛觉过敏的影响及可能的机制.方法:选用Sprague-Dawley (SD)雄性大鼠30只,选用L5脊神经切断神经病理性模型；随机分成3组(n=l0):假手术+生理盐水组、损伤+生理盐水组、损伤+重组人促红细胞生成素(recombinant human erythropoietin,rhEPO) (5000 U/kg)组；术前1天给药,连续给药7天.采用von Frey纤维和Hargreaves法测定各组机械缩足反射阈值(mechanical withdrawal threshold,MWT)和热缩足潜伏期(thermal withdrawal latency,TWL)；采用实时定量多聚酶链反应(real-time polymerase chain reaction,RT-PCR)的方法检测脊髓胶质细胞标志物白细胞分化抗原11b (cluster of differentiation antigen 11b,CD11b)和胶质细胞酸性蛋白(glial fibrillary acidic protein,GFAP)的mRNA表达水平.结果:与生理盐水预给药手术组相比,预防性腹腔注射rhEPO 5000 U/kg,能显著缓解大鼠的机械、热痛高敏行为(P＜0.05,P＜0.01),降低脊髓胶质细胞白细胞分化抗原和胶质细胞酸性蛋白的mRNA表达水平(P＜0.05).结论:发现预防性给予rhEPO能预防神经病理性疼痛的发生.其效应与其抑制胶质细胞活性相关.
목적:탐토예방성급여중조인홍세포생성소대웅성대서L5척신경절단대서궤계、열통각과민적영향급가능적궤제.방법:선용Sprague-Dawley (SD)웅성대서30지,선용L5척신경절단신경병이성모형；수궤분성3조(n=l0):가수술+생리염수조、손상+생리염수조、손상+중조인촉홍세포생성소(recombinant human erythropoietin,rhEPO) (5000 U/kg)조；술전1천급약,련속급약7천.채용von Frey섬유화Hargreaves법측정각조궤계축족반사역치(mechanical withdrawal threshold,MWT)화열축족잠복기(thermal withdrawal latency,TWL)；채용실시정량다취매련반응(real-time polymerase chain reaction,RT-PCR)적방법검측척수효질세포표지물백세포분화항원11b (cluster of differentiation antigen 11b,CD11b)화효질세포산성단백(glial fibrillary acidic protein,GFAP)적mRNA표체수평.결과:여생리염수예급약수술조상비,예방성복강주사rhEPO 5000 U/kg,능현저완해대서적궤계、열통고민행위(P＜0.05,P＜0.01),강저척수효질세포백세포분화항원화효질세포산성단백적mRNA표체수평(P＜0.05).결론:발현예방성급여rhEPO능예방신경병이성동통적발생.기효응여기억제효질세포활성상관.