食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
11期
3603-3609
,共7页
高夫超%崔长日%魏月%吴黎明
高伕超%崔長日%魏月%吳黎明
고부초%최장일%위월%오려명
高效液相色谱法%蜂王浆%糠醛类物质
高效液相色譜法%蜂王漿%糠醛類物質
고효액상색보법%봉왕장%강철류물질
high performance liquid chromatography%royal jelly%furfural compounds
目的:建立同时测定蜂王浆中5-羟甲基-2-糠醛(5-HMF)、5-甲基糠醛(5-MF)、2-糠醛(F)、2-糠酸(2-OIC)、3-糠酸(3-OIC)、5-羟甲基-2-糠酸(5-HMFacid)等6种糠醛类物质含量的高效液相色谱分析方法。方法样品经去离子水提取,亚铁氰化钾和乙酸锌溶液沉淀蛋白后,用C18色谱柱分离,0.5%甲酸水溶液和甲醇梯度洗脱,在各化合物的最佳吸收波长处进行检测。结果在测试范围内,6种糠醛类物质的浓度与相应的峰面积呈线性关系,相关系数(R2)不小于0.9993。方法定量和定性检出限分别在0.07~0.26μg/mL和0.02~0.08μg/mL之间,加标回收率为86.2%~99.7%,相对标准偏差小于2.40%。结论该方法操作简单,灵敏度高,适合蜂王浆中6种糠醛类化合物的测定。
目的:建立同時測定蜂王漿中5-羥甲基-2-糠醛(5-HMF)、5-甲基糠醛(5-MF)、2-糠醛(F)、2-糠痠(2-OIC)、3-糠痠(3-OIC)、5-羥甲基-2-糠痠(5-HMFacid)等6種糠醛類物質含量的高效液相色譜分析方法。方法樣品經去離子水提取,亞鐵氰化鉀和乙痠鋅溶液沉澱蛋白後,用C18色譜柱分離,0.5%甲痠水溶液和甲醇梯度洗脫,在各化閤物的最佳吸收波長處進行檢測。結果在測試範圍內,6種糠醛類物質的濃度與相應的峰麵積呈線性關繫,相關繫數(R2)不小于0.9993。方法定量和定性檢齣限分彆在0.07~0.26μg/mL和0.02~0.08μg/mL之間,加標迴收率為86.2%~99.7%,相對標準偏差小于2.40%。結論該方法操作簡單,靈敏度高,適閤蜂王漿中6種糠醛類化閤物的測定。
목적:건립동시측정봉왕장중5-간갑기-2-강철(5-HMF)、5-갑기강철(5-MF)、2-강철(F)、2-강산(2-OIC)、3-강산(3-OIC)、5-간갑기-2-강산(5-HMFacid)등6충강철류물질함량적고효액상색보분석방법。방법양품경거리자수제취,아철청화갑화을산자용액침정단백후,용C18색보주분리,0.5%갑산수용액화갑순제도세탈,재각화합물적최가흡수파장처진행검측。결과재측시범위내,6충강철류물질적농도여상응적봉면적정선성관계,상관계수(R2)불소우0.9993。방법정량화정성검출한분별재0.07~0.26μg/mL화0.02~0.08μg/mL지간,가표회수솔위86.2%~99.7%,상대표준편차소우2.40%。결론해방법조작간단,령민도고,괄합봉왕장중6충강철류화합물적측정。
Objective To establish a reversed-phase high performance liquid chromatography method for determining 5-hydroxymethyl-2-furfural (5-HMF), 5-methyl-2- furaldehyde (5-MF), 2-furaldehyde (F), fu-ran-2-carboxylic acid (2-OIC), furan-3-carboxylic acid (3-OIC) and 5-hydroxymethyl-2-furancarboxylic acid (5-HMF acid) in royal jelly (RJ).Methods Samples were extracted with purity water and proteins in them were removed by zinc acetate and potassium ferrocyanide solutions. Before injection, the solutions were passed through 0.45μm filters for determination by a liquid chromatography UV detection at different wavelengths. Results Within the test ranges, 6 furfural compounds concentrations and their peak areas showed good linear correlations (R2≥0.9993). The limits of quantitation and detection were 0.07~0.26μg/mL and 0.02~0.08μg/mL, respectively. The recoveries ranged from 86.2% to 99.7% and the overall relative standards were lower than 2.40%.ConclusionThe method is simple, sensitive and suitable for analyzing 6 furfural com-pounds in RJ.