CAJ | 학술논문

以无纺布为固定化载体,以Candida rugosa脂肪酶为催化剂,催化植物甾醇与油酸酯化反应合成植物甾醇油酸酯。研究了反应温度、反应时间、底物摩尔比(油酸与植物甾醇摩尔比)、正己烷用量和酶用量对植物甾醇酯化率的影响。在单因素实验的基础上,经响应面实验优化反应条件。结果表明,植物甾醇油酸酯最佳合成条件为：反应温度50℃,反应时间18 h,底物摩尔比2∶1,正己烷用量1 mL,酶用量62.06 U/mg。在最佳条件下,植物甾醇酯化率可达98.36%。
이무방포위고정화재체,이Candida rugosa지방매위최화제,최화식물치순여유산지화반응합성식물치순유산지。연구료반응온도、반응시간、저물마이비(유산여식물치순마이비)、정기완용량화매용량대식물치순지화솔적영향。재단인소실험적기출상,경향응면실험우화반응조건。결과표명,식물치순유산지최가합성조건위：반응온도50℃,반응시간18 h,저물마이비2∶1,정기완용량1 mL,매용량62.06 U/mg。재최가조건하,식물치순지화솔가체98.36%。
With non-woven fabric as immobilized carrier,esterification of phytosterols and oleic acid was catalyzed by Candida rugosa lipase. The effects of reaction temperature,reaction time,molar ratio of sub-strate ( molar ratio of oleic acid to phytosterols) ,n-hexane dosage and enzyme dosage on esterification rate of phytosterols were studied. Based on single factor experiment,the optimal synthesis conditions of phytosterol oleate were obtained by response surface methodology as follows:reaction temperature 50℃, reaction time 18 h,molar ratio of substrate 2∶1,n-hexane dosage 1 mL,enzyme dosage 62. 06 U/mg. Under the optimal conditions,the esterification rate of phytosterols could reach 98. 36%.