CAJ | 학술논문

研究了二十二碳六烯酸-磷脂( DHA-磷脂)对实验性肥胖小鼠脂质代谢的调节作用及其机制。采用Folch法和硅胶柱层析法从鸢乌贼( Sthenoteuthis oualaniensis )卵中分离制备得到富含DHA的磷脂。 C57 BL/6 J雄性小鼠随机分为3组：高脂模型组,1%大豆磷脂组,1%DHA-磷脂组。连续饲喂7周后,测定小鼠血清总胆固醇( TC)、甘油三酯( TG)、高密度脂蛋白胆固醇( HDL-C)含量,肝脏TC、TG、磷脂(PL)含量以及肝脏脂肪合成酶(FAS)、葡萄糖-6-磷酸脱氢酶(G6PDH)、苹果酸酶( ME)、磷脂酸磷酸酶( PAP)、肉毒碱棕榈酸转移酶( CPT)、过氧化物酶体β-氧化活力。结果显示,DHA-磷脂能极显著抑制小鼠体重( P<0．01)和肾周脂肪( P<0．01)及精周脂肪( P<0．01)蓄积,降低血清TC(P<0．01)和肝脏TG(P<0．01)水平,抑制肝脏FAS(P<0．01)、ME(P<0．05)、PAP(P<0．05)的活力,提高CPT(P<0．01)和过氧化物酶体β-氧化(P<0．05)的活力,且效果优于大豆磷脂。 DHA-磷脂具有良好的减肥作用,能够通过抑制肝脏脂质合成,促进脂质分解,有效调节肥胖小鼠的脂质代谢。
연구료이십이탄륙희산-린지( DHA-린지)대실험성비반소서지질대사적조절작용급기궤제。채용Folch법화규효주층석법종연오적( Sthenoteuthis oualaniensis )란중분리제비득도부함DHA적린지。 C57 BL/6 J웅성소서수궤분위3조：고지모형조,1%대두린지조,1%DHA-린지조。련속사위7주후,측정소서혈청총담고순( TC)、감유삼지( TG)、고밀도지단백담고순( HDL-C)함량,간장TC、TG、린지(PL)함량이급간장지방합성매(FAS)、포도당-6-린산탈경매(G6PDH)、평과산매( ME)、린지산린산매( PAP)、육독감종려산전이매( CPT)、과양화물매체β-양화활력。결과현시,DHA-린지능겁현저억제소서체중( P<0．01)화신주지방( P<0．01)급정주지방( P<0．01)축적,강저혈청TC(P<0．01)화간장TG(P<0．01)수평,억제간장FAS(P<0．01)、ME(P<0．05)、PAP(P<0．05)적활력,제고CPT(P<0．01)화과양화물매체β-양화(P<0．05)적활력,차효과우우대두린지。 DHA-린지구유량호적감비작용,능구통과억제간장지질합성,촉진지질분해,유효조절비반소서적지질대사。
The effects of docosahexaenoic acid-enriched phospholipids ( DHA-PL) on lipid metabolism in diet-induced obese C57BL/6J mice model were investigated. DHA-PL were extracted from Stheno-teuthis oualaniensis spawns by Folch method and purified by silica gel column chromatography. The mice were assigned to three groups randomly: high-fat model group, 1% soybean phospholipids group and 1% DHA-PL group. After seven weeks of feeding, the contents of triglycerides ( TG) , total cholesterol ( TC) and high density lipoprotein cholesterol ( HDL-C) in serum and the contents of TC, TG and PL in liver of the mice were meassured, and the activities of hepatic fatty acid synthase(FAS), malic en-zyme(ME), glucose-6-phosphate dehydrogenase(G6PDH), carnitine palmitoyl transterase (CPT), peroxisomal β-oxidation enzymes and phosphatidic acid phophyhydrolase ( PAP) were determined. The results showed that DHA-PL inhibited the accumulations of body weight ( P<0. 01 ) , perirenal adipose tissue(P<0. 01) and epididymal adipose tissue (P<0. 01) of mice significantly, reduced the contents of TC ( P <0. 01 ) in serum and hepatic TG ( P< 0. 01 ) , inhibited the activities of FAS ( P<0. 01 ) , ME ( P <0. 05 ) and PAP ( P <0. 05) in liver markedly, and enhanced the ac-tivities of CPT ( P < 0. 01 ) and peroxisomalβ-oxidation enzymes ( P<0. 05 ) . The effect of DHA - PL on improving the lipid metabolism was superior to that of soybean phospholipids.DHA-PL played a good role in weight loss, and it could promote decomposition of lipid through inhibi-ting synthesis of lipid in liver and regulate lipid metabolism of obese mice effectively.