CAJ | 학술논문

在无溶剂体系中,通过脂肪酶Novozyme 435催化甘油解法合成功能性油脂1,3-甘油二酯。在底物摩尔比1∶1(甘油与天然谷物调和油比),脂肪酶添加量8%(占底物质量)条件下,研究酶催化反应过程中不同HLB值的蔗糖脂肪酸酯和反应温度对1,3-甘油二酯生成量的影响。研究结果表明,选择HLB 值为11的亲水性蔗糖脂肪酸酯,在添加量0.5%,反应温度60℃下反应24 h,1,3-甘油二酯生成量最高,为42.61%。
재무용제체계중,통과지방매Novozyme 435최화감유해법합성공능성유지1,3-감유이지。재저물마이비1∶1(감유여천연곡물조화유비),지방매첨가량8%(점저물질량)조건하,연구매최화반응과정중불동HLB치적자당지방산지화반응온도대1,3-감유이지생성량적영향。연구결과표명,선택HLB 치위11적친수성자당지방산지,재첨가량0.5%,반응온도60℃하반응24 h,1,3-감유이지생성량최고,위42.61%。
The functional lipid 1 ,3-diacylglycerol was synthesized in free-solvent system by glycerolysis with Novozyme 435 as catalyst. Under the conditions of molar ratio of substrates 1∶1 ( ratio of glycerol to natural grain blend oil) and lipase dosage 8% ( based on the mass of substrates) , the effects of sucrose fatty acid esters with different HLB values and reaction temperature on the yield of 1 ,3 - diacylglycerol were studied. The results showed that under the conditions of hydrophilic sucrose fatty acid esters ( HLB value 11) dosage 0. 5%, reaction temperature 60℃, reaction time 24 h, the yield of 1,3-diacylglycer-ol was up to 42 . 61%.