CAJ | 학술논문

荧光量子点由于具有发射荧光效率高、光稳定性好、无光漂白等优良特性，因此在生物学研究中得到广泛应用。用量子点标记细胞，通过量子点荧光可以对细胞进行长时程观察，实现活细胞动态示踪。利用量子点和DiO荧光染料对A549细胞膜双染，直观展示了量子点优于有机荧光染料的抗光漂白特性，并阐述了一种利用量子点对细胞A549进行标记并观测在α粒子辐照下细胞损伤动态变化过程的方法，这种方法对于阐明粒子辐射作用细胞的过程、了解辐射损伤的机理有一定的优势。
형광양자점유우구유발사형광효솔고、광은정성호、무광표백등우량특성，인차재생물학연구중득도엄범응용。용양자점표기세포，통과양자점형광가이대세포진행장시정관찰，실현활세포동태시종。이용양자점화DiO형광염료대A549세포막쌍염，직관전시료양자점우우유궤형광염료적항광표백특성，병천술료일충이용양자점대세포A549진행표기병관측재α입자복조하세포손상동태변화과정적방법，저충방법대우천명입자복사작용세포적과정、료해복사손상적궤리유일정적우세。
Owing to the excellent optical properties such as high quantum yield of emission, photo-stability and anti-photobleaching, fluorescent quantum dots(QDs) now have been extensively applied in bioscience re-search. Labeling cells by quantum dots allow long-term observation of the cellular processes. In this paper, we employed both QDs and DiO dye to label cells and thus showed the superior anti-photobleaching property of QDs directly, and also reported the method of application of QDs in observing the damage process of A549 cells induced byα particle irradiation, demonstrating the advantage of application of QDs in the radiobiology research.