甘肃农业大学学报
甘肅農業大學學報
감숙농업대학학보
JOURNAL OF GANSU AGRICULTURAL UNIVERSITY
2014年
4期
7-11
,共5页
赵书艺%高欣%方梅%贾宁
趙書藝%高訢%方梅%賈寧
조서예%고흔%방매%가저
藏羊%巴氏杆菌%细菌学实验%病理学
藏羊%巴氏桿菌%細菌學實驗%病理學
장양%파씨간균%세균학실험%병이학
Tibetan sheep%pasteurellosis%bacteria assay%pathology
为了对甘肃省武威市某羊场藏羊自然爆发的疑似巴氏杆菌病的病羊进行确诊和病理学研究,试验采用染色镜检、分离培养、生化试验鉴定、动物试验、病理组织制作和染色等方法,对采集的病料分别进行了细菌学检测和病理组织学观察。结果发现:瑞氏染色可见两端着色椭圆形短杆菌,分离培养、生化试验鉴定、动物试验均表明其特性为多杀性巴氏杆菌。病理组织学变化为:肺脏呈纤维素性-化脓性肺炎,心脏呈程度不等的变质性心肌炎和化脓性心肌炎,肝脏呈程度不等变质性肝炎,脾脏呈程度不等坏死,肾脏呈急性肾小球肾炎和局灶性化脓性肾炎,全身淋巴结呈浆液-出血性淋巴结炎。同时,病羊重要实质器官肝、脾、肾、淋巴结等组织内均出现大量单核巨噬细胞的广泛增生。病变组织中间质胶原纤维肿胀、断裂、崩解,血管壁纤维素样坏死。结果表明,本次武威市某羊场藏羊自然爆发疾病为羊巴氏杆菌病,其主要病理组织学变化特点为肺脏呈纤维素性-化脓性肺炎和心脏的变质性-化脓性心肌炎。
為瞭對甘肅省武威市某羊場藏羊自然爆髮的疑似巴氏桿菌病的病羊進行確診和病理學研究,試驗採用染色鏡檢、分離培養、生化試驗鑒定、動物試驗、病理組織製作和染色等方法,對採集的病料分彆進行瞭細菌學檢測和病理組織學觀察。結果髮現:瑞氏染色可見兩耑著色橢圓形短桿菌,分離培養、生化試驗鑒定、動物試驗均錶明其特性為多殺性巴氏桿菌。病理組織學變化為:肺髒呈纖維素性-化膿性肺炎,心髒呈程度不等的變質性心肌炎和化膿性心肌炎,肝髒呈程度不等變質性肝炎,脾髒呈程度不等壞死,腎髒呈急性腎小毬腎炎和跼竈性化膿性腎炎,全身淋巴結呈漿液-齣血性淋巴結炎。同時,病羊重要實質器官肝、脾、腎、淋巴結等組織內均齣現大量單覈巨噬細胞的廣汎增生。病變組織中間質膠原纖維腫脹、斷裂、崩解,血管壁纖維素樣壞死。結果錶明,本次武威市某羊場藏羊自然爆髮疾病為羊巴氏桿菌病,其主要病理組織學變化特點為肺髒呈纖維素性-化膿性肺炎和心髒的變質性-化膿性心肌炎。
위료대감숙성무위시모양장장양자연폭발적의사파씨간균병적병양진행학진화병이학연구,시험채용염색경검、분리배양、생화시험감정、동물시험、병리조직제작화염색등방법,대채집적병료분별진행료세균학검측화병리조직학관찰。결과발현:서씨염색가견량단착색타원형단간균,분리배양、생화시험감정、동물시험균표명기특성위다살성파씨간균。병리조직학변화위:폐장정섬유소성-화농성폐염,심장정정도불등적변질성심기염화화농성심기염,간장정정도불등변질성간염,비장정정도불등배사,신장정급성신소구신염화국조성화농성신염,전신림파결정장액-출혈성림파결염。동시,병양중요실질기관간、비、신、림파결등조직내균출현대량단핵거서세포적엄범증생。병변조직중간질효원섬유종창、단렬、붕해,혈관벽섬유소양배사。결과표명,본차무위시모양장장양자연폭발질병위양파씨간균병,기주요병리조직학변화특점위폐장정섬유소성-화농성폐염화심장적변질성-화농성심기염。
The tissue samples from pasteurellosis suspected Tibet sheep were tested by pathological method in Wuwei City,Gansu Province.The result of bacterium analysis showed that the disease pathogen of the Tibetan sheep was Pasteurellamultocida.The diseases of the Tibetan sheep were diagnosed as pas-teurellosis.The pathological changes indicate following characteristics:the lungs emerge fibrinous pneu-monia and suppurative pneumonia mixture performance.The hearts have varying degrees myocarditis.The livers emerge varying degrees hepatitis.The spleens appear acute splenitis.The kidneys have viously degen-eration and acute glomerulonephritis and suppurative nephritis.The lymph nodes emerge serious acute lym-phadenitis.At the same time,there are large number of macrophages and multiple nuclear giant cells to in-tersperse in livers,kidneys,spleens and lymph nodes.The blood vessels of important tissues occurred fi-brinoid necrosis.The collagen fibers and elastic fibers are swelled and collapsed in this tissues.