CAJ | 학술논문

采用实时荧光定量PCR技术检测了A-FABP 和CAPN 1基因在‘黑羽番鸭’肌肉生长发育过程中 mR-N A表达规律。结果显示：在胸肌中,A-FABP 基因在2周龄时表达最高；在腿肌中,A-FABP 基因在8周龄时表达量最高。在胸肌、腿肌组织中A-FABP 基因mRNA的表达量大致为2~4周龄下降,4~8周龄上升,8~13周龄再次出现下降,公母鸭的变化趋势一致。在胸肌、腿肌中,CAPN 1基因在2周龄时表达量最高。在胸肌组织中, CAPN1基因mRNA的表达量在2~10周龄之间下降,10~13周龄上升。在腿肌组织中,CAPN1基因 mRNA 的表达量先下降,后上升,最后再次下降。
채용실시형광정량PCR기술검측료A-FABP 화CAPN 1기인재‘흑우번압’기육생장발육과정중 mR-N A표체규률。결과현시：재흉기중,A-FABP 기인재2주령시표체최고；재퇴기중,A-FABP 기인재8주령시표체량최고。재흉기、퇴기조직중A-FABP 기인mRNA적표체량대치위2~4주령하강,4~8주령상승,8~13주령재차출현하강,공모압적변화추세일치。재흉기、퇴기중,CAPN 1기인재2주령시표체량최고。재흉기조직중, CAPN1기인mRNA적표체량재2~10주령지간하강,10~13주령상승。재퇴기조직중,CAPN1기인 mRNA 적표체량선하강,후상승,최후재차하강。
In this study,mRNA expression regulation of A-FABP and CAPN1 genes,following the growing development of muscle,was detected by Real-Time PCR in black Muscovy duck.The results indi-cated that the highest A-FABP mRNA expression in breast muscle was detected at 2-week-old,and in leg muscle was detected at 8-week-old.In the tissue of breast muscle and leg muscle,the A-FABP mRNA ex-pression decreased from 2-week to 4-week,and increased from 4-week to 8-week,and decreased again from 8-week to 1 3-week.The changes were the same in sexes.The highest CAPN1 mRNA expression was detec-ted in breast muscle at 2-week-old,and the same result in leg muscle.In the tissue of breast muscle,the CAPN1 mRNA expression decreased from 2-week to 10-week,and increased from 10-week to 13-week.In the tissue of leg muscle,the CAPN1 mRNA expression decreased first,and then increased,and decreased at last.