CAJ | 학술논문

目的：探讨氟尿嘧啶(5-FU)和奥沙利铂(OX)在胃癌中的体外化疗敏感性。方法：纳入病理确诊并自愿接受检测的胃癌患者,采用ATP肿瘤化疗敏感性检测法( ATP-TCA)检测新鲜手术标本对5-FU和OX的体外化疗敏感性。结果：纳入27例,男性21例、女性6例,中位年龄60．0岁,远侧部胃癌14例(51．9%),低分化及印戒细胞癌共20例(74．1%),TNM Ⅲ+Ⅳ期21例(77．8%)。 ATP-TCA检测显示5-FU+OX在各药物浓度的肿瘤生长抑制率均显著高于5-FU或OX(P<0．05),5-FU+OX的抑制曲线下面积显著高于5-FU或OX(P<0．05)；而其化疗敏感指数、IC90和IC50显著低于5-FU或OX ( P<0．05)。5-FU、OX和5-FU+OX的体外化疗敏感性依次为18．5%、14．8%和55．6%,5-FU+OX的敏感性显著高于5-FU或OX(5-FU+OX vs．5-FU：P=0．001和5-FU+OX vs． OX：P=0．003)。 Logistic回归分析显示5-FU的敏感性与临床病理特征无显著相关性,而OX和5-FU+OX的敏感性分别与Borrmann分型(OR=7．570,P=0．025)和肿瘤位置(OR=3．427,P=0．019)有显著相关性。结论：5-FU+OX在胃癌中显示出较高的体外化疗敏感性,但其与体内疗效的相关性有待进一步临床观察。
목적：탐토불뇨밀정(5-FU)화오사리박(OX)재위암중적체외화료민감성。방법：납입병리학진병자원접수검측적위암환자,채용ATP종류화료민감성검측법( ATP-TCA)검측신선수술표본대5-FU화OX적체외화료민감성。결과：납입27례,남성21례、녀성6례,중위년령60．0세,원측부위암14례(51．9%),저분화급인계세포암공20례(74．1%),TNM Ⅲ+Ⅳ기21례(77．8%)。 ATP-TCA검측현시5-FU+OX재각약물농도적종류생장억제솔균현저고우5-FU혹OX(P<0．05),5-FU+OX적억제곡선하면적현저고우5-FU혹OX(P<0．05)；이기화료민감지수、IC90화IC50현저저우5-FU혹OX ( P<0．05)。5-FU、OX화5-FU+OX적체외화료민감성의차위18．5%、14．8%화55．6%,5-FU+OX적민감성현저고우5-FU혹OX(5-FU+OX vs．5-FU：P=0．001화5-FU+OX vs． OX：P=0．003)。 Logistic회귀분석현시5-FU적민감성여림상병리특정무현저상관성,이OX화5-FU+OX적민감성분별여Borrmann분형(OR=7．570,P=0．025)화종류위치(OR=3．427,P=0．019)유현저상관성。결론：5-FU+OX재위암중현시출교고적체외화료민감성,단기여체내료효적상관성유대진일보림상관찰。
Objective: To investigate the in vitro chemosensitivity of fluorouracil (5-FU) and oxaliplatin (OX) in gastric cancer. Methods:Patients with histologically confirmed gastric cancer were enrolled for the study. All patients a-greed to the chemosensitivity test of their resected tumors and gave informed consent. Adenosine triphosphate tumor chemo-sensitivity assay ( ATP-TCA) was performed to determine the chemosensitivity of 5-FU and OX in resected gastric cancer specimens. Results:Twenty-seven patients with a median age of 60. 0 were enrolled, including 21 males and 6 females. The tumors were located in the lower part of the stomach in 14 cases, 20 cases were poorly differentiated or signet-ring cell carcinoma and 21 cases were in late TNM stage. ATP-TCA assay showed that the tumor growth inhibition rates of 5-FU+OX were significantly higher than those of 5-FU or OX at each test drug concentration ( P<0. 05 ); correspondingly, the area under the curve of 5-FU+OX was significantly higher than those of 5-FU or OX (P<0. 05), whereas their chemosen-sitivity index, IC90 and IC50 were significantly lower than those of 5-FU or OX (P<0. 05). The chemosensitivity rates of 5-FU, OX and 5-FU+OX were 18. 5%, 14. 8% and 55. 6%, respectively; chemosensitivity of 5-FU+OX was signifi-cantly higher than that of 5-FU or OX (5-FU+OX vs. 5-FU:P=0. 001 and 5-FU+OX vs. OX:P=0. 003). Logistic re-gression analysis indicated that the chemosensitivity of 5-FU was not significantly correlated with any clinicopathological characteristics;however, the chemosensitivity of OX and 5-FU+OX was significantly correlated with Borrmann type ( OR=7. 570, P=0. 025) and tumor location (OR=3. 427, P=0. 019), respectively. Conclusion:5-FU+OX showed high in vitro chemosensitivity in gastric cancer, and may be worthy of further exploration to see its correlation with in vivo efficacy.