CAJ | 학술논문

基于固态培养基，对1株由传统醋酸发酵醋醅分离筛选所得醋酸菌A10的扩大培养进行了研究。研究了接种量、培养温度、固态培养基酸酒比、培养过程中翻醅的频率对醋酸菌扩大培养的影响，并进行了正交试验。实验结果表明：接种量6％，培养温度32℃，酸酒比1．4∶1，间隔12 h 翻醅1次，醋酸菌A10平均24 h增值率达67％。
기우고태배양기，대1주유전통작산발효작배분리사선소득작산균A10적확대배양진행료연구。연구료접충량、배양온도、고태배양기산주비、배양과정중번배적빈솔대작산균확대배양적영향，병진행료정교시험。실험결과표명：접충량6％，배양온도32℃，산주비1．4∶1，간격12 h 번배1차，작산균A10평균24 h증치솔체67％。
Based on the solid medium,the expanding cultivation of acetic acid bacteria A10 isolated from traditional acetic acid fermentation of vinegar fermented grains is studied.The effects of acetic acid bacteria inoculum size,incubation temperature,ratio of acid and alcohol before inoculation,sti-ring frequency on expanding cultivation of acetic acid bacteria are studied by orthogonal experiment. The results show that when acetic acid bacteria inoculum size is 6%,inoculation temperature is 32 ℃, ratio of acid and alcohol is 1 .4∶1 ,stirring frequency is 12 h,the average growth rate of acetic acid bacteria A10 is 67% in 24 h.