中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2013年
21期
86-89
,共4页
谢丽艳%徐洁%戴国梁%居文政%谈恒山
謝麗豔%徐潔%戴國樑%居文政%談恆山
사려염%서길%대국량%거문정%담항산
消癌平注射液%新绿原酸%原儿茶醛%绿原酸%隐绿原酸%香草酸%咖啡酸%4-香酸
消癌平註射液%新綠原痠%原兒茶醛%綠原痠%隱綠原痠%香草痠%咖啡痠%4-香痠
소암평주사액%신록원산%원인다철%록원산%은록원산%향초산%가배산%4-향산
Xiaoaiping injection%neochlorogenic acid%protocatechuic aldehyde%chlorogenic acid%4-dicaffeoylquinic acid%vanillic acid%caffeic acid%4-coumararate
目的:测定消癌平注射液中新绿原酸、原儿茶醛、绿原酸、隐绿原酸、咖啡酸、香草酸和4-香豆素的含量.方法:Kromasil 100-5 C18色谱柱(4.6 mm×250mm,5μm),流动相乙腈-0.2％磷酸,梯度洗脱,检测波长300 nm;柱温30℃,流速0.8mL·min-1.结果:新绿原酸、原儿茶醛、绿原酸、隐绿原酸、咖啡酸、香草酸和4-香豆酸的线性范围分别为0.1 ～3.2 mg·L-1(r=0.999 3)、0.025 ～0.8 mg·L-1(r =0.999 9),0.1 ～3.2 mg·L-1(r =0.999 8),0.075 ～2.4 mg·L-1(r =0.999 9),0.037 5 ～1.2 mg·L-1(r =0.999 9),0.02～0.64 mg·L-1(r =0.999 9),0.01 ～0.32 mg·L-1(r =0.999 9),加样回收率分别为99.6％(RSD 0.24％),100.0％ (RSD 0.15％),98.7％ (RSD 1.00％),99.1％ (RSD 1.36％),96.4％ (RSD 1.37％),98.3％ (RSD1.80％),97.3％ (RSD 1.64％).结论:该方法简便,准确,重复性好,可为消癌平注射液提供质量控制依据.
目的:測定消癌平註射液中新綠原痠、原兒茶醛、綠原痠、隱綠原痠、咖啡痠、香草痠和4-香豆素的含量.方法:Kromasil 100-5 C18色譜柱(4.6 mm×250mm,5μm),流動相乙腈-0.2％燐痠,梯度洗脫,檢測波長300 nm;柱溫30℃,流速0.8mL·min-1.結果:新綠原痠、原兒茶醛、綠原痠、隱綠原痠、咖啡痠、香草痠和4-香豆痠的線性範圍分彆為0.1 ～3.2 mg·L-1(r=0.999 3)、0.025 ～0.8 mg·L-1(r =0.999 9),0.1 ～3.2 mg·L-1(r =0.999 8),0.075 ～2.4 mg·L-1(r =0.999 9),0.037 5 ～1.2 mg·L-1(r =0.999 9),0.02～0.64 mg·L-1(r =0.999 9),0.01 ～0.32 mg·L-1(r =0.999 9),加樣迴收率分彆為99.6％(RSD 0.24％),100.0％ (RSD 0.15％),98.7％ (RSD 1.00％),99.1％ (RSD 1.36％),96.4％ (RSD 1.37％),98.3％ (RSD1.80％),97.3％ (RSD 1.64％).結論:該方法簡便,準確,重複性好,可為消癌平註射液提供質量控製依據.
목적:측정소암평주사액중신록원산、원인다철、록원산、은록원산、가배산、향초산화4-향두소적함량.방법:Kromasil 100-5 C18색보주(4.6 mm×250mm,5μm),류동상을정-0.2％린산,제도세탈,검측파장300 nm;주온30℃,류속0.8mL·min-1.결과:신록원산、원인다철、록원산、은록원산、가배산、향초산화4-향두산적선성범위분별위0.1 ～3.2 mg·L-1(r=0.999 3)、0.025 ～0.8 mg·L-1(r =0.999 9),0.1 ～3.2 mg·L-1(r =0.999 8),0.075 ～2.4 mg·L-1(r =0.999 9),0.037 5 ～1.2 mg·L-1(r =0.999 9),0.02～0.64 mg·L-1(r =0.999 9),0.01 ～0.32 mg·L-1(r =0.999 9),가양회수솔분별위99.6％(RSD 0.24％),100.0％ (RSD 0.15％),98.7％ (RSD 1.00％),99.1％ (RSD 1.36％),96.4％ (RSD 1.37％),98.3％ (RSD1.80％),97.3％ (RSD 1.64％).결론:해방법간편,준학,중복성호,가위소암평주사액제공질량공제의거.
