CAJ | 학술논문

以采自峨眉山的桢楠叶和带叶芽的茎为外植体，MS为基础培养基，组合4种不同激素配比的培养基，进行愈伤组织诱导实验，结果表明：（1）4种不同植物激素配比的培养基，都能促使桢楠不同部位的外植体诱导形成愈伤组织，但诱导愈伤组织形成情况具有较明显的差异。（2）以MS为基础培养基，添加2?0mg/L NAA＋2?0mg/L 6-BA，是愈伤组织诱导的最佳培养基。（3）以叶为外植体诱导形成愈伤组织的能力强于以带叶芽的茎段，形成的愈伤组织具有颜色偏绿、质地紧密、褐化少等特点，将愈伤组织转接到增殖培养基上，具有增殖能力。
이채자아미산적정남협화대협아적경위외식체，MS위기출배양기，조합4충불동격소배비적배양기，진행유상조직유도실험，결과표명：（1）4충불동식물격소배비적배양기，도능촉사정남불동부위적외식체유도형성유상조직，단유도유상조직형성정황구유교명현적차이。（2）이MS위기출배양기，첨가2?0mg/L NAA＋2?0mg/L 6-BA，시유상조직유도적최가배양기。（3）이협위외식체유도형성유상조직적능력강우이대협아적경단，형성적유상조직구유안색편록、질지긴밀、갈화소등특점，장유상조직전접도증식배양기상，구유증식능력。
In this study,Phoebe zhennan leaves and stems with buds collected from Emei Mountain were selected as explants,and MS medium combined with four different ratio of hormones were used to conduct callus induction experiments.The results showed that all explants of Phoebe zhennan in this study can be in-duced with callus for the four different ratio of culture medium,but the induction effect of callus has signifi-cant difference among the different parts;the best medium for callus induction is MS medium adding 2.0 mg/L NAA and 2.0mg/L 6-BA;callus induced by leaf as explant is much easier than leaf bud stems,if transferred to proliferation medium,the callus has the ability of proliferation.