CAJ | 학술논문

以成熟中苎1号叶片为材料，采用Percoll密度梯度离心法，分离获得完整的叶绿体，并用植物基因组DNA提取试剂盒提取叶绿体DNA，经核酸和蛋白分析仪、琼脂糖电泳、核基因组及叶绿体基因组特异引物PCR检测，结果表明：所提取的苎麻叶绿体DNA纯度高，不含核基因组DNA，并适用于基因扩增等分子操作，为深入研究苎麻叶绿体基因组奠定基础。同时，本方法操作简便、快速，适用性广，为其它物种叶绿体DNA的提取提供借鉴。
이성숙중저1호협편위재료，채용Percoll밀도제도리심법，분리획득완정적협록체，병용식물기인조DNA제취시제합제취협록체DNA，경핵산화단백분석의、경지당전영、핵기인조급협록체기인조특이인물PCR검측，결과표명：소제취적저마협록체DNA순도고，불함핵기인조DNA，병괄용우기인확증등분자조작，위심입연구저마협록체기인조전정기출。동시，본방법조작간편、쾌속，괄용성엄，위기타물충협록체DNA적제취제공차감。
Undamaged chloroplast was isolated by using Percoll differential and density -gradient centrifugation from leaf tissues of ramie ( Boehmeria nivea ( L.) Gaud.) , Zhongzhu No.1.And then, Plant Genomic DNA Kit was used to extract chloroplast DNA.The detection results of nucleic acid pro-tein analyzer , agrose gel electrophoresis and PCR amplification based on chloroplast and nuclear genome specific primers showed that ramie cpDNA with high purity and quality , and no nuclear genome DNA might provide a foundation for further study of chloroplast genome in ramie.The DNA was suitable for be-ing used as template in molecular experiment researches such as PCR reaction , gene cloning and so on.The method is rapid , simple and also suitable for isolating cpDNA from many other plants.