CAJ | 학술논문

为了提高红麻愈伤组织不定芽诱导率，以红麻子叶和胚轴为外植体，采用两种途径诱导红麻不定芽分化，对影响红麻不定芽分化的培养基组成因素进行研究。结果表明：（1）在诱导愈伤组织分化不定芽的途径中，两种外植体均可诱导产生愈伤组织，但是胚轴产生的愈伤组织质量优于子叶。在分化培养基MS＋6-BA （1mg/L）＋2，4-D （1mg/L）＋GA3（1mg/L）中，愈伤组织可分化少量不定芽，但是分化率较低；当该分化培养基中添加0.3％的活性炭时，愈伤组织在分化不定芽的同时也产生了大量不定根。（2）在通过直接诱导外植体分化不定芽的途径中，胚轴难以诱导产生不定芽，子叶可有效诱导不定芽；在MS＋TDZ （0.1 mg/L ）＋NAA （0.1mg/L）的诱导培养基中，子叶的不定芽诱导效果最好，诱导率为64.29％。而在诱导培养基中添加 AgNO3后，不定芽的诱导率明显提高，其诱导效果最好的培养基为 MS ＋TDZ （0.1mg/L）＋NAA （0.1mg/L）＋AgNO3（1mg/L），此时不定芽诱导率可达85％。
위료제고홍마유상조직불정아유도솔，이홍마자협화배축위외식체，채용량충도경유도홍마불정아분화，대영향홍마불정아분화적배양기조성인소진행연구。결과표명：（1）재유도유상조직분화불정아적도경중，량충외식체균가유도산생유상조직，단시배축산생적유상조직질량우우자협。재분화배양기MS＋6-BA （1mg/L）＋2，4-D （1mg/L）＋GA3（1mg/L）중，유상조직가분화소량불정아，단시분화솔교저；당해분화배양기중첨가0.3％적활성탄시，유상조직재분화불정아적동시야산생료대량불정근。（2）재통과직접유도외식체분화불정아적도경중，배축난이유도산생불정아，자협가유효유도불정아；재MS＋TDZ （0.1 mg/L ）＋NAA （0.1mg/L）적유도배양기중，자협적불정아유도효과최호，유도솔위64.29％。이재유도배양기중첨가 AgNO3후，불정아적유도솔명현제고，기유도효과최호적배양기위 MS ＋TDZ （0.1mg/L）＋NAA （0.1mg/L）＋AgNO3（1mg/L），차시불정아유도솔가체85％。
Cotyledon and hypocotyl were used as explants to study the influential factors of kenaf ad -ventitious buds differentiation through two approaches.The results showed that:( 1 ) in the approach of callus induction , the quality of hypocotyl callus was better than cotyledon , callus could be induced to dif-ferentiate a few adventitious buds in the medium of MS +6-BA (1mg/L) +2, 4-D (1mg/L) +GA3 (1mg/L), but the induction rate was very low.After adding 0.3%activated carbon in the medi-um, many roots were induced along with adventitious buds.(2) In the approach of inducing explants di-rectly, hypocotyl hardly generated adventitious buds , cotyledon could induce adventitious effectively.Cotyledon could get the highest induction rate in the medium of MS +TDZ ( 0.1 mg/L ) +NAA (0.1mg/L), and the rate was 64.29%.After adding AgNO3 in the medium, the rate could increase ob-viously.The best induction medium was MS +TDZ (0.1mg/L) +NAA (0.1mg/L) +AgNO3 (1mg/L) , and the adventitious buds induction rate was 85%.