中国麻业科学
中國痳業科學
중국마업과학
PLANT FIBER SCIENCES IN CHINA
2013年
5期
221-225
,共5页
黄思齐%陈艳翠%李建军%唐慧娟%陈安国%李德芳
黃思齊%陳豔翠%李建軍%唐慧娟%陳安國%李德芳
황사제%진염취%리건군%당혜연%진안국%리덕방
红麻%质核互作雄性不育%功能分析
紅痳%質覈互作雄性不育%功能分析
홍마%질핵호작웅성불육%공능분석
kenaf%cytoplasmic-nuclear male sterility%gene analysis
根据得到的atp1基因序列设计CDS区特异引物,分别以红麻不育系和保持系的DNA和RNA反转录得到的cDNA为模板扩增atp1基因,测序并进行序列比对后发现,以DNA为模板和以cDNA为模板扩增得到的基因序列在不育系和保持系间序列完全一致,这暗示不育性状并非碱基变异造成。同时,使用半定量RT-PCR技术较全面地分析了atp1基因在红麻中的转录表达特征,结果显示: atp1基因在不育系和保持系的根、茎、叶以及花瓣、花药、雌蕊中都表达,但在不育系的叶片和花药中表达下降,暗示造成不育的原因可能与atp1基因的表达量下降有关。研究为下一步构建表达载体进行转基因表达研究提供了基础,为揭示雄性不育相关基因导致雄性不育的机理提供一定依据。
根據得到的atp1基因序列設計CDS區特異引物,分彆以紅痳不育繫和保持繫的DNA和RNA反轉錄得到的cDNA為模闆擴增atp1基因,測序併進行序列比對後髮現,以DNA為模闆和以cDNA為模闆擴增得到的基因序列在不育繫和保持繫間序列完全一緻,這暗示不育性狀併非堿基變異造成。同時,使用半定量RT-PCR技術較全麵地分析瞭atp1基因在紅痳中的轉錄錶達特徵,結果顯示: atp1基因在不育繫和保持繫的根、莖、葉以及花瓣、花藥、雌蕊中都錶達,但在不育繫的葉片和花藥中錶達下降,暗示造成不育的原因可能與atp1基因的錶達量下降有關。研究為下一步構建錶達載體進行轉基因錶達研究提供瞭基礎,為揭示雄性不育相關基因導緻雄性不育的機理提供一定依據。
근거득도적atp1기인서렬설계CDS구특이인물,분별이홍마불육계화보지계적DNA화RNA반전록득도적cDNA위모판확증atp1기인,측서병진행서렬비대후발현,이DNA위모판화이cDNA위모판확증득도적기인서렬재불육계화보지계간서렬완전일치,저암시불육성상병비감기변이조성。동시,사용반정량RT-PCR기술교전면지분석료atp1기인재홍마중적전록표체특정,결과현시: atp1기인재불육계화보지계적근、경、협이급화판、화약、자예중도표체,단재불육계적협편화화약중표체하강,암시조성불육적원인가능여atp1기인적표체량하강유관。연구위하일보구건표체재체진행전기인표체연구제공료기출,위게시웅성불육상관기인도치웅성불육적궤리제공일정의거。
Specific primers of CDS were designed according to CDS region predicted.The atp1 genes were amplified using DNA and cDNA as template in kenaf male sterile line and its maintainer line respec-tively.The sequences of atp1 gene amplified from DNA and cDNA had no difference between kenaf male sterile line and its maintainer line by sequencing and sequence alignment.It suggested that the sterility was not caused by base mutation of atp1 gene.Semi-RT-PCR analysis indicated that atp1 gene ex-pressed in root, stem, leaf, petal, anther and pistil, but the expression level in leaf and anther of male sterile line was lower than maintainer line , which suggested that the male sterility in kenaf was possibly related to the expression decrease of atp1 gene.