医学检验与临床
醫學檢驗與臨床
의학검험여림상
MEDICAL LATORATORY SCIENCE AND CLINICES
2013年
5期
37-39,89
,共4页
流感嗜血杆菌%肺炎链球菌%耐药性%β-内酰胺酶%青霉素
流感嗜血桿菌%肺炎鏈毬菌%耐藥性%β-內酰胺酶%青黴素
류감기혈간균%폐염련구균%내약성%β-내선알매%청매소
Haemophilus influenzae%Streptococcus pneumoniae%Resistance%β-lactamase enzyme%Penicill
目的：了解临床分离流感嗜血杆菌和肺炎链球菌的分布及耐药情况，为临床合理使用抗菌药物、预防和控制感染提供依据。方法：收集医院2005~2012年各类临床标本分离流感嗜血杆菌92株、肺炎链球菌83株，均经全自动细菌鉴定仪鉴定，用K-B法检测流感嗜血杆菌对常用的15种抗生素及肺炎链球菌对常见的13种抗生素的敏感性，并用头孢硝噻吩纸片法检测流感嗜血杆菌产β-内酰胺酶情况。结果：流感嗜血杆菌和肺炎链球菌主要来源于呼吸道标本，不同季节流感嗜血杆菌的感染率不同，冬春两季为高发季节，肺炎链球菌感染患者的年龄呈双峰分布，以年龄＜5岁和＞50岁的感染者最多。流感嗜血杆菌对氨苄西林、复方新诺明和头孢呋辛的耐药率较高，产β-内酰胺酶检出率为64.17%。肺炎链球菌对红霉素、氯霉素、四环素、克林霉素耐药性非常严重，其中47株（56.63%）为青霉素不敏感肺炎链球菌（PNSP）。结论：流感嗜血杆菌和肺炎链球菌耐药情况较为严重，对流感嗜血杆菌和肺炎链球菌的耐药性进行严密监测具有重要意义。
目的：瞭解臨床分離流感嗜血桿菌和肺炎鏈毬菌的分佈及耐藥情況，為臨床閤理使用抗菌藥物、預防和控製感染提供依據。方法：收集醫院2005~2012年各類臨床標本分離流感嗜血桿菌92株、肺炎鏈毬菌83株，均經全自動細菌鑒定儀鑒定，用K-B法檢測流感嗜血桿菌對常用的15種抗生素及肺炎鏈毬菌對常見的13種抗生素的敏感性，併用頭孢硝噻吩紙片法檢測流感嗜血桿菌產β-內酰胺酶情況。結果：流感嗜血桿菌和肺炎鏈毬菌主要來源于呼吸道標本，不同季節流感嗜血桿菌的感染率不同，鼕春兩季為高髮季節，肺炎鏈毬菌感染患者的年齡呈雙峰分佈，以年齡＜5歲和＞50歲的感染者最多。流感嗜血桿菌對氨芐西林、複方新諾明和頭孢呋辛的耐藥率較高，產β-內酰胺酶檢齣率為64.17%。肺炎鏈毬菌對紅黴素、氯黴素、四環素、剋林黴素耐藥性非常嚴重，其中47株（56.63%）為青黴素不敏感肺炎鏈毬菌（PNSP）。結論：流感嗜血桿菌和肺炎鏈毬菌耐藥情況較為嚴重，對流感嗜血桿菌和肺炎鏈毬菌的耐藥性進行嚴密鑑測具有重要意義。
목적：료해림상분리류감기혈간균화폐염련구균적분포급내약정황，위림상합리사용항균약물、예방화공제감염제공의거。방법：수집의원2005~2012년각류림상표본분리류감기혈간균92주、폐염련구균83주，균경전자동세균감정의감정，용K-B법검측류감기혈간균대상용적15충항생소급폐염련구균대상견적13충항생소적민감성，병용두포초새분지편법검측류감기혈간균산β-내선알매정황。결과：류감기혈간균화폐염련구균주요래원우호흡도표본，불동계절류감기혈간균적감염솔불동，동춘량계위고발계절，폐염련구균감염환자적년령정쌍봉분포，이년령＜5세화＞50세적감염자최다。류감기혈간균대안변서림、복방신낙명화두포부신적내약솔교고，산β-내선알매검출솔위64.17%。폐염련구균대홍매소、록매소、사배소、극림매소내약성비상엄중，기중47주（56.63%）위청매소불민감폐염련구균（PNSP）。결론：류감기혈간균화폐염련구균내약정황교위엄중，대류감기혈간균화폐염련구균적내약성진행엄밀감측구유중요의의。
Objective:To investigate the characteristics of distribution and drug resistance of Haemophilus influenzae and Streptococcus pneumoniae, provide the basis of using antimicrobial drugs for clinical. Methods:92 clinical isolates of Haemophilus influenzae and 83 isolates of Streptococcus pneumoniae were collected during 2003 to 2012, and then identified by Vitek-2 system. K-B disk method was used to test antimicrobial susceptibility.β-lactamase was detected with nitrocefin disk testing.Results:92 Haemophilus influenzae and 83 Streptococcus pneumoniae of clinical isolates mainly from respiratory, The higher infectious season of Haemophilus influenza was winter and spring. The age of the Streptococcus pneumoniae infection patient is bimodal distribution, mainly<5 years old and>50 years old. The resistance rates of Haemophilus influenzae to ampicillin、cotrimoxazole and cefuroxime were high, and 64.17%Haemophilus influenzae producedβ-lactamase. The resistance rates of Streptococcus pneumoniae to erythromycin、chloramphenicol、tetracycline、clindamycin were serious, and there were 47 (56.63%) isolates of Streptococcus pneumoniae were penicillin non-susceptible (PNSP). Conclusions:The resistance of Haemophilus influenzae and Streptococcus pneumoniae were serious. Monitoring the resistance of Haemophilus influenzae and Streptococcus pneumoniae will help to reduce the generation of resistant strains.
