CAJ | 학술논문

基于过氧化氢与鲁米诺化学发光反应，并应用MPI-F型流动注射化学发光仪分析测定呋喃西林含量的方法。该方法的最佳测定条件：H2O20.10mol/l，NaOH0.10mol/l，鲁米诺5.0×10-6mol/l，负高压为800V，蠕动泵主泵运行10s后副泵运行30s，接着主泵继续转动10s，副泵30s，如此循环，上述化学发光强度因呋喃西林的存在而显著减弱，且其强度的减弱值（ΔI）与呋喃西林浓度在0.0500-2.00μg/ml之间呈线性关系，方法的检出限为0.0100μg/ml。对0.100μg/ml的呋喃西林进行11次平行测定，其相对标准偏差为6.50%。该法已成功测定了三种不同浓度呋喃西林精粉的含量，其相对标准偏差分别为2.01%、1.25%、3.50%，平均回收率在98.8%-100.5%之间。
기우과양화경여로미낙화학발광반응，병응용MPI-F형류동주사화학발광의분석측정부남서림함량적방법。해방법적최가측정조건：H2O20.10mol/l，NaOH0.10mol/l，로미낙5.0×10-6mol/l，부고압위800V，연동빙주빙운행10s후부빙운행30s，접착주빙계속전동10s，부빙30s，여차순배，상술화학발광강도인부남서림적존재이현저감약，차기강도적감약치（ΔI）여부남서림농도재0.0500-2.00μg/ml지간정선성관계，방법적검출한위0.0100μg/ml。대0.100μg/ml적부남서림진행11차평행측정，기상대표준편차위6.50%。해법이성공측정료삼충불동농도부남서림정분적함량，기상대표준편차분별위2.01%、1.25%、3.50%，평균회수솔재98.8%-100.5%지간。
the experiment in this paper is based on the chemiluminescence emission produced upon mixing a hydrogen peroxide solution with an alkaline luminol solution and a MPI-F flow-injection chemiluminescence instrument was employed as well. The optimum condition for this method is described as below:H2O20.1mol/1, NaOH0.1mol/1, luminol 5.0x10-6mol/1, negative high voltage 800V, main peristaltic pump runs for 10s and entailed by sub-peristaltic pump for 30s and this cycle is repeated;the intensity of chemiluminescence was apparently weakened due to the existence of Furacilin, and the weakening value (△I) has a linear relation with the content of Furacilin at 0.0500-2.00μg/mL, the determination limit of this method is 0.0100μg/mL. 11 parallel measurements were carried out upon 0.0100μg/mL Furacilin, the relative standard deviation is 6.50%. This method has successfully measured the content of Furacilin of three different condensations, the relative deviation were 2.01%, 1.25% and 3.50%respectively;the average recycling rates are between 98.8%and 100.5%.