CAJ | 학술논문

利用采自山东章丘、济宁表现典型粗缩病症状的玉米植株样品进行ELISA和RT-PCR检测。间接ELISA结果表明，二者均与水稻黑条矮缩病毒(RBSDV)多克隆抗体表现阳性反应。RT-PCR扩增出该病毒基因组S10约1.8 kb片段。获得的1801 bp全长序列与已发表的相关序列进行同源性分析和系统进化树分析，结果表明，章丘分离物克隆JNZ-14序列、济宁分离物克隆JN-8序列均属于RBSDV，但其与玉米粗缩病毒(MRDV)的进化关系比其与南方水稻黑条矮缩病毒(SRBSDV)的关系还要近，且2个分离物分别处于属于RBSDV的Ⅱ或Ⅰ两大分支中。
이용채자산동장구、제저표현전형조축병증상적옥미식주양품진행ELISA화RT-PCR검측。간접ELISA결과표명，이자균여수도흑조왜축병독(RBSDV)다극륭항체표현양성반응。RT-PCR확증출해병독기인조S10약1.8 kb편단。획득적1801 bp전장서렬여이발표적상관서렬진행동원성분석화계통진화수분석，결과표명，장구분리물극륭JNZ-14서렬、제저분리물극륭JN-8서렬균속우RBSDV，단기여옥미조축병독(MRDV)적진화관계비기여남방수도흑조왜축병독(SRBSDV)적관계환요근，차2개분리물분별처우속우RBSDV적Ⅱ혹Ⅰ량대분지중。
Typical rough dwarf symptom from Zhangqiu and Jining of Shandong was detected by ELISA and RT-PCR methods. The results from indirect ELISA indicated that, both the samples showed positive reaction with RBSDV polyclonal antibody. RT-PCR amplified about 1.8 kb segment of the viral genome S10. the 1.8 kb full-length sequence acquired had made homology and phylogenetic tree analysis with published sequences related. The results showed that, the Zhangqiu isolate clones JNZ-14 sequence, the Jining isolate clones, JN-8, belongs to Rice black-streaked dwarf virus(RBSDV),but the evolutionary relationship with MRDV were even closer than theirs with southern rice black-streaked dwarf virus(SRBSDV), moreover, the two isolates were in largeⅡorⅠbranches of RBS-DV respectively.