CAJ | 학술논문

针对草甘膦结合位点，对高粱5-烯醇式丙酮酰莽草酸-3-磷酸合酶(EPSPS)基因进行4种定点修饰，将修饰后的基因分别导入到玉米中。通过对转化体的抗性鉴定，确定将高粱EPSPS基因106位的脯氨酸变为丝氨酸(P106S)能够赋予转基因玉米草甘膦抗性。在喷施4倍的草甘膦时抗性事件CL38-1不产生药害。 Southern杂交结果表明，目标基因在该转化事件中稳定遗传，转化其余3种EPSPS基因的植株对草甘膦没有足够的抗性。
침대초감련결합위점，대고량5-희순식병동선망초산-3-린산합매(EPSPS)기인진행4충정점수식，장수식후적기인분별도입도옥미중。통과대전화체적항성감정，학정장고량EPSPS기인106위적포안산변위사안산(P106S)능구부여전기인옥미초감련항성。재분시4배적초감련시항성사건CL38-1불산생약해。 Southern잡교결과표명，목표기인재해전화사건중은정유전，전화기여3충EPSPS기인적식주대초감련몰유족구적항성。
According to the potential binding site of glyphosate, Sorghum 5-enolpyruvylshikimate-3-phosphate synthase(SbEPSPS) and conducted four kinds of site-directed modification were cloned. These modified SbEPSPS were introduced in maize using transgenic approach. We found that the transgenic maize with one amino acid substi-tution: proline(P106) to serine(S) of EPSPS gained glyphosate resistance. One of transgenic events CL38-1 showed glyphosate resistant after sprayed with 4-fold glyphosate. Southern blot analysis of transformant showed that the trans-gene inherited stably. Plants transformed with other three types of modified SbEPSPS genes did not show enough glyphosate resistance.