生物技术进展
生物技術進展
생물기술진전
2013年
6期
421-426
,共6页
钟葵%佟立涛%刘丽娅%周闲容%钟昕%周素梅
鐘葵%佟立濤%劉麗婭%週閒容%鐘昕%週素梅
종규%동립도%류려아%주한용%종흔%주소매
裂褶多糖%固体发酵%辐照%免疫调节活性%抗肿瘤活性
裂褶多糖%固體髮酵%輻照%免疫調節活性%抗腫瘤活性
렬습다당%고체발효%복조%면역조절활성%항종류활성
schizophyllan%solid-state fermentation%irradiation%immunity-modulatory activity%antitumor activity
以麦麸和玉米芯为原料固体发酵生产裂褶多糖（schizophyllan，SPG），采用辐照技术降解处理SPG（irradiated schizophyllan，ISPG），研究辐照前后裂褶多糖分子量、体内免疫调节及抗肿瘤活性变化。结果显示，辐照能有效降低天然SPG分子量，分子量从106～107 Da（ SPG）降低到104～105 Da（ ISPG）。 SPG和ISPG均具有良好免疫调节和抗肿瘤活性， SPG和ISPG中剂量（100 mg/kg体重·d）和低剂量（50 mg/kg体重·d）组均对S180荷瘤小鼠羊红细胞诱导迟发型变态反应（ DTH）和脾细胞介导羊红细胞定量溶血值（ QHS）具有显著增强功效，ISGP中剂量组（100 mg/kg体重·d）能显著增强小鼠巨噬细胞吞噬鸡红细胞效果，提升小鼠非特异性免疫功能；SPG和ISPG不同剂量组抑瘤率均在45％以上，ISPG中剂量组（100 mg/kg体重·d）抑瘤率最高（74．80％±6．83％）。总体看来，辐照处理后的ISPG比天然SPG具有更好的免疫调节及抗肿瘤活性。
以麥麩和玉米芯為原料固體髮酵生產裂褶多糖（schizophyllan，SPG），採用輻照技術降解處理SPG（irradiated schizophyllan，ISPG），研究輻照前後裂褶多糖分子量、體內免疫調節及抗腫瘤活性變化。結果顯示，輻照能有效降低天然SPG分子量，分子量從106～107 Da（ SPG）降低到104～105 Da（ ISPG）。 SPG和ISPG均具有良好免疫調節和抗腫瘤活性， SPG和ISPG中劑量（100 mg/kg體重·d）和低劑量（50 mg/kg體重·d）組均對S180荷瘤小鼠羊紅細胞誘導遲髮型變態反應（ DTH）和脾細胞介導羊紅細胞定量溶血值（ QHS）具有顯著增彊功效，ISGP中劑量組（100 mg/kg體重·d）能顯著增彊小鼠巨噬細胞吞噬鷄紅細胞效果，提升小鼠非特異性免疫功能；SPG和ISPG不同劑量組抑瘤率均在45％以上，ISPG中劑量組（100 mg/kg體重·d）抑瘤率最高（74．80％±6．83％）。總體看來，輻照處理後的ISPG比天然SPG具有更好的免疫調節及抗腫瘤活性。
이맥부화옥미심위원료고체발효생산렬습다당（schizophyllan，SPG），채용복조기술강해처리SPG（irradiated schizophyllan，ISPG），연구복조전후렬습다당분자량、체내면역조절급항종류활성변화。결과현시，복조능유효강저천연SPG분자량，분자량종106～107 Da（ SPG）강저도104～105 Da（ ISPG）。 SPG화ISPG균구유량호면역조절화항종류활성， SPG화ISPG중제량（100 mg/kg체중·d）화저제량（50 mg/kg체중·d）조균대S180하류소서양홍세포유도지발형변태반응（ DTH）화비세포개도양홍세포정량용혈치（ QHS）구유현저증강공효，ISGP중제량조（100 mg/kg체중·d）능현저증강소서거서세포탄서계홍세포효과，제승소서비특이성면역공능；SPG화ISPG불동제량조억류솔균재45％이상，ISPG중제량조（100 mg/kg체중·d）억류솔최고（74．80％±6．83％）。총체간래，복조처리후적ISPG비천연SPG구유경호적면역조절급항종류활성。
Schizophyllan ( SPG) was produced by the fungus Schizophyllum commune under solid-state fermentation conditions in this study. Irradiation technology was used to treat SPG and molecular weight and biological effects of SPG and irradiated schizophyllan ( ISPG) on the immunity-modulatory and antitumor activities in S180 tumor mice models in vivo were investigated. Irradiation technology decreased significantly the molecular weight of SPG ( P< 0.05) , and the range of molecular weight of SPG and ISPG were 106~107 Da and 104~105 Da, respectively. Both SPG and ISPG with medium dose ( 100 mg/kg) and low dose ( 50 mg/kg) had potent immune-modulatory effects in S180 tumor mice model and exhibited significant effect on delayed-type hypersensitivity (DTH) response and quantitative hemolysis spectrophotometry (QHS) test (P<0.05). Besides, ISPG with low dose (50 mg/kg) elevated significantly the phagocytosis of macrophage (P<0.05). The results still showed that SPG and ISPG had great antitumor effects, and ISPG at medium dose ( 100 mg/kg ) had maximum inhibition rate ( 74. 80%± 6. 83%) . Therefore, ISPG had better immunity-modulatory and antitumor activities than natural SPG.
