CAJ | 학술논문

精液的冷冻保存是辅助生殖技术的基础，但精液冻融过程会产生过多的活性氧自由基（ROS），破坏精子的生理结构，使其质量下降，造成受精能力下降。目前，有些研究开始关注抗氧化剂在精液冷冻保存过程中的保护作用。其中一些来源于植物的天然抗氧化剂（如白藜芦醇、槲皮苷、染料木黄酮等），这些物质的来源广泛，抗氧化能力非常高，而且毒性较低，可以有效抑制精子冻融后ROS的产生，减少精子在冷冻过程中受到的氧化损伤，从而保护精子的活力和遗传稳定性，可以作为冷冻保护剂的添加成份用于改良精子的冷冻保存。
정액적냉동보존시보조생식기술적기출，단정액동융과정회산생과다적활성양자유기（ROS），파배정자적생리결구，사기질량하강，조성수정능력하강。목전，유사연구개시관주항양화제재정액냉동보존과정중적보호작용。기중일사래원우식물적천연항양화제（여백려호순、곡피감、염료목황동등），저사물질적래원엄범，항양화능력비상고，이차독성교저，가이유효억제정자동융후ROS적산생，감소정자재냉동과정중수도적양화손상，종이보호정자적활력화유전은정성，가이작위냉동보호제적첨가성빈용우개량정자적냉동보존。
Sperm cryopreservation technique is a basis of assisted reproduction. However,the additional reactive oxygen species (ROS) was produced in the freezing-thawing process,which damaged the physiological structure and function of spermatozoa,and lowered sperm quality and fertility. At present,there were some studies on the protective roles of antioxidants in the sperm cryopreservation. Some natural antioxidants derived from plants are widespread,high efficient and low toxicity. These natural antioxidants can significantly inhibit ROS production,eliminate the sperm damage caused by ROS,and protect sperm activity and genetic stability. In future,these natural antioxidants may be used as additional ingredients in cryoprotective agent for improving sperm cryopreservation.