CAJ | 학술논문

目的：分析胶质瘤中核干细胞因子（NS）表达和异柠檬酸脱氢酶1（IDHI）基因突变的特点，为胶质瘤的诊断、治疗提供理论依据。方法对58例原发性胶质瘤存档蜡块分析，采用免疫组织化学法检测 NS 蛋白的表达情况，应用 PCR技术检测标本中IDH1基因多态性变化。结果免疫组织化学法检测显示，在高级别胶质瘤标本中NS蛋白表达率高于低级别。经基因直接测序，16例（55.2％）出现IDH1突变，突变位点均为R132型。经统计学分析，IDH1基因突变率与胶质瘤病理分级呈正相关。结论在胶质瘤中核干细胞因子表达和ID H l基因突变率随病理级别的增加而增加，可为胶质瘤的诊断提供有力的证据，并为胶质瘤在基因治疗方面指出了新的研究方向。
목적：분석효질류중핵간세포인자（NS）표체화이저몽산탈경매1（IDHI）기인돌변적특점，위효질류적진단、치료제공이론의거。방법대58례원발성효질류존당사괴분석，채용면역조직화학법검측 NS 단백적표체정황，응용 PCR기술검측표본중IDH1기인다태성변화。결과면역조직화학법검측현시，재고급별효질류표본중NS단백표체솔고우저급별。경기인직접측서，16례（55.2％）출현IDH1돌변，돌변위점균위R132형。경통계학분석，IDH1기인돌변솔여효질류병리분급정정상관。결론재효질류중핵간세포인자표체화ID H l기인돌변솔수병리급별적증가이증가，가위효질류적진단제공유력적증거，병위효질류재기인치료방면지출료신적연구방향。
Objective To analyze the characteristic of expression of NS and isocitrate dehydrogenase (IDH1) gene muta-tions in gliomas ,as to provide theoretical basis for the diagnosis and treatment of glioma. Methods Fifty-eight human glioma samples were analyzed. The protein expression levels of NS were measured by IHC. The polymorphisms change of IDH1 gene was detected by real-time quantitative PCR.Results Upshots immuno-histochemistry manifested that the positive expression rate of NS in high level gloma tissue was higher than that in low level glioma. Gene sequencing manifested that IDH1 gene mu-tations were observed in 16 samples having gliomas(55.2% ) ,and the mutation appeared as R132H type. By the SPSS analy-sis ,IDH1 gene mutation rate had a certain correlation with glioma pathology classification.Conclusion As the increase of path-ological level ,the expression of NS and IDH1 gene mutations rate are increased in glioma. It can provide strong evidence for the diagnosis of glioma and opened up a new research way in gene therapy for gliomas.