CAJ | 학술논문

[目的]应用间接免疫荧光法（IFA）检测出现呼吸道症状且腋温≥37．3℃的新兵流感病毒 A/B （Flu A、B）抗体，探讨其快速检测 Flu A、B 抗体的可行性，调查呼吸道感染且发热患者 Flu A、B 抗体的水平。[方法]收集北京郊区某部队5个营区中出现咳嗽、咳痰、流涕、咽痛等呼吸道症状且腋温≥37．3℃的94名新兵的血清标本，应用 IFA 检测 Flu A、B 抗体，分析检测结果。[结果]Flu A、B IgM 抗体阳性率分别为2．1％、8．5％；北方籍出现呼吸道症状且发热的新兵人数明显高于南方籍新兵、沿海明显高于内陆，差异均有统计学意义（P ＜0．05），各营区间差异无统计学意义（P ＞0．05）；南北方籍、沿海和内陆、各营区间新兵恢复期 Flu B IgM 抗体差异均无统计学意义（P ＞0．05）。[结论]流感病毒感染不容忽视，IFA 法检测操作简单、便捷，适合在流感病毒流行时大规模快速筛查患者。
[목적]응용간접면역형광법（IFA）검측출현호흡도증상차액온≥37．3℃적신병류감병독 A/B （Flu A、B）항체，탐토기쾌속검측 Flu A、B 항체적가행성，조사호흡도감염차발열환자 Flu A、B 항체적수평。[방법]수집북경교구모부대5개영구중출현해수、해담、류체、인통등호흡도증상차액온≥37．3℃적94명신병적혈청표본，응용 IFA 검측 Flu A、B 항체，분석검측결과。[결과]Flu A、B IgM 항체양성솔분별위2．1％、8．5％；북방적출현호흡도증상차발열적신병인수명현고우남방적신병、연해명현고우내륙，차이균유통계학의의（P ＜0．05），각영구간차이무통계학의의（P ＞0．05）；남북방적、연해화내륙、각영구간신병회복기 Flu B IgM 항체차이균무통계학의의（P ＞0．05）。[결론]류감병독감염불용홀시，IFA 법검측조작간단、편첩，괄합재류감병독류행시대규모쾌속사사환자。
[Objective]To detect the antibody level of influenza virus A(Flu A)and B(Flu B)in recruits with respiratory symptoms and axillary temperature ≥ 37.3℃ by indirect immunofluorescence assay(IFA), and to explore the feasibility of rapid detection of Flu A and B antibodies,and to investigate the level of Flu A and B antibodies in patients with respiratory infections and fever.[Methods]Serum samples were collected from 94 recruits with cough,sputum,runny nose,sore throat and other respiratory symptoms and axillary temperature≥37.3℃ in five camps around Beijing.Flu A and B antibodies were detected by IFA.The test re-sults were analyzed.[Results]Positive rate of IgM antibody of Flu A and B were 2.1% and 8.5%,respec-tively.The northern recruits with respiratory symptoms and fever were significantly higher than southern re-cruits,and recruits in coastal areas were significantly higher than those in inland areas,and there were signifi-cant differences(P <0.05),but there was no significant difference between different military camps.There was no significant difference in IgM antibody of Flu B in recruits during recovery period in northern,southern, coastal and inland areas and different military camps(P >0.05).[Conclusion]Influenza virus infection can not be ignored.IFA method is simple,convenient and suitable for rapid screening of patients in the epidemics of influenza virus.