CAJ | 학술논문

目的：研究三七总皂苷对脑出血大鼠凝血酶及神经功能影响。方法：SD雄性大鼠200只随机分为空白组、假手术组、模型组、用药组，每组又有6 h，24 h，48 h，72 h，7天5个时间点。空白组每组5只，其余每组每个时间点10只大鼠。采用尾壳核注入胶原酶肝素生理盐水溶液法建立大鼠脑出血模型，治疗组给予三七总皂苷腹腔注射，每天一次。在相应时间点，采用改良的神经功能缺损评分（ Modified neurological severity score ，mNSS）对实验动物造模后各时间点神经功能缺损进行评价。结果：各时间点模型组mNSS和空白组假手术组比，明显升高，差异有统计学意（P＜0.05），除6 h外，各时间点模型组和用药组比，用药组mNSS明显降低，差异有统计学意义（ P＜0.05）。测定纤维蛋白原含量和纤维蛋白原凝血时间，纤维蛋白原含量和纤维蛋白原凝血时间48 h模型组和用药组差异有统计学意义（ P＜0.05）。结论：脑出血模型组中，6 h开始，mNSS明显增高，到48 h达到高峰，以后逐渐下降，三七总皂苷可以明显下调mNSS评分。纤维蛋白原含量和纤维蛋白原凝血时间在模型组升高，用药组下降，说明三七发挥了发挥了活血行淤作用。
목적：연구삼칠총조감대뇌출혈대서응혈매급신경공능영향。방법：SD웅성대서200지수궤분위공백조、가수술조、모형조、용약조，매조우유6 h，24 h，48 h，72 h，7천5개시간점。공백조매조5지，기여매조매개시간점10지대서。채용미각핵주입효원매간소생리염수용액법건립대서뇌출혈모형，치료조급여삼칠총조감복강주사，매천일차。재상응시간점，채용개량적신경공능결손평분（ Modified neurological severity score ，mNSS）대실험동물조모후각시간점신경공능결손진행평개。결과：각시간점모형조mNSS화공백조가수술조비，명현승고，차이유통계학의（P＜0.05），제6 h외，각시간점모형조화용약조비，용약조mNSS명현강저，차이유통계학의의（ P＜0.05）。측정섬유단백원함량화섬유단백원응혈시간，섬유단백원함량화섬유단백원응혈시간48 h모형조화용약조차이유통계학의의（ P＜0.05）。결론：뇌출혈모형조중，6 h개시，mNSS명현증고，도48 h체도고봉，이후축점하강，삼칠총조감가이명현하조mNSS평분。섬유단백원함량화섬유단백원응혈시간재모형조승고，용약조하강，설명삼칠발휘료발휘료활혈행어작용。
Objective:To investigate the effects of Panax notoginseng saponins ( PNS) on the cerebral edema and thrombin in the rats with cerebral hemorrhage (ICH).Methods:In present study, 200 SD rats were randomly divided into 4 groups: blank control, sham control , model and PNS treatment groups .ICH was induced by stereotactic injection of the collagenase Ⅳinto the right caudate nucleus . PNS or saline was injected intraperitoneally once a day after ICH , and the neurologic impairment was assessed by nMSS method for 5 time points:6 h, 24 h, 48 hour, 72 h and 7 d.Both content of serous fibrinogen (FIB) and Fibrinogen clotting time (FIBs) were measured.Results:The score of mNSS increased from 6h (P<0.05) following ICH, and peaked at 48h, then returned to the normal on the 7th day, and the PNS decreased cerebral water content significantly at every examined time points except for 6 h (P<0.05). Moreover, both serous FIB and FIBs increased obviously at 48 h after ICH (P<0.05).The PNS inhibited the increase of both content compared with that in model group (FIB P=0.017, TT P=0.028).Conclusion:In rats, the score of mNSS started from 6h following ICH with a peak at 48 hours, and then decreased gradually , accompanied by the elevation of FIB and FIBs at 48h.PNS can effectively alleviate the score of mNSS via the inhibition of coagulation cascade pathway .