CAJ | 학술논문

目的：探讨血府逐瘀汤对人脐静脉内皮细胞一氧化氮（Nitric oxide，NO）产生的影响。方法：以人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cell -12，HUVEC-12）为材料，采用不同血府逐瘀汤含药血清浓度和干预时间处理HUVEC-12，检测细胞培养上清液中NO浓度的变化。结果：与空白对照组对比，血府逐瘀汤可在一定干预时间段，适度诱导HUVEC-12胞外NO的产生（ P＜0.05）。结论：血府逐瘀汤可能通过适度刺激人内皮细胞NO产生，发挥促进血管新生作用，又避免增生过度的不良反应。
목적：탐토혈부축어탕대인제정맥내피세포일양화담（Nitric oxide，NO）산생적영향。방법：이인제정맥내피세포（Human Umbilical Vein Endothelial Cell -12，HUVEC-12）위재료，채용불동혈부축어탕함약혈청농도화간예시간처리HUVEC-12，검측세포배양상청액중NO농도적변화。결과：여공백대조조대비，혈부축어탕가재일정간예시간단，괄도유도HUVEC-12포외NO적산생（ P＜0.05）。결론：혈부축어탕가능통과괄도자격인내피세포NO산생，발휘촉진혈관신생작용，우피면증생과도적불량반응。
Objective:To investigate effects of Xuefu Zhuyu Decoction ( XFZYD) on levels of Nitric oxide ( NO) released from Human Umbilical Vein Endothelial cells .Methods:Endothelial cells HUVEC-12 were incubated with blank serum or XFZYD containing serum (XFZYD-CS) at different concentrations and different times .Thereafter, the NO concentrations in the culture supernatant liquid were determined .Results:Compared with the control group , XFZYD-CS has a moderate stimulatory effect on NO production of this endotheli-al cell line in a period of time .Conclusion:Xuefu Zhuyu Decoction moderately increases the endothelial NO concentration to promote angiogenesis without causing overgrown blood vessels .