CAJ | 학술논문

对核桃楸树皮粗粉进行提取分离，鉴定所得产物并优化提取工艺。将核桃楸药材粗粉，经乙醇回流提取，乙酸乙酯和氯仿萃取，后经反复硅胶柱层析梯度洗脱得到3种黄酮类化合物。以正交试验考查最佳提取工艺。通过正交试验得到提取核桃楸树皮有效成分的最佳工艺为以8倍核桃楸粗粉质量的75％乙醇回流提取3次，每次历时1.5 h，并用核磁共振的方法对所得化合物进行鉴定。乙醇回流提取并分离得到核桃楸树皮中的杨梅素、柚皮素、橙皮素3种黄酮类单体是可行的。
대핵도추수피조분진행제취분리，감정소득산물병우화제취공예。장핵도추약재조분，경을순회류제취，을산을지화록방췌취，후경반복규효주층석제도세탈득도3충황동류화합물。이정교시험고사최가제취공예。통과정교시험득도제취핵도추수피유효성분적최가공예위이8배핵도추조분질량적75％을순회류제취3차，매차력시1.5 h，병용핵자공진적방법대소득화합물진행감정。을순회류제취병분리득도핵도추수피중적양매소、유피소、등피소3충황동류단체시가행적。
To optimize the extraction process of effective compositions from Cortex Juglandis Mandshuricae.Cortex Juglandis Mandshuricae were extracted with ethanol,concentrated with ethyl acetate and chloroform and eluted gradiently on silica gel to get 3 kinds of flavonoids compounds.Orthogonal experiments were used to examine the best extraction process.The best extraction process is to use 75% ethanol with 8 times the weight of Cortex Juglandis Mandshuricae scoured grist to reflux extract 3 times with 1.5 h each time.The products identified by nuclear magnetic resonance meth-ods.Myricetin, naringenin and hesperitin are the main contents of the Cortex Juglandis Mandshuricae, and the ethanol refluxing extraction process is feasible.