CAJ | 학술논문

使用不同浓度的氯化镉（ CdCl2）处理体外培养的条斑星鲽卵巢（ BFO）细胞系细胞，利用毒理学和细胞生物学方法研究了重金属镉对BFO细胞的细胞毒性及其作用机理。毒性作用研究结果显示，BFO细胞对CdCl2敏感，浓度大于10μmol/L的CdCl2对细胞的生长具有显著的抑制作用，能引起细胞内超氧化物歧化酶（ SOD）、谷胱甘肽过氧化物酶（ GST-Px）活性的持续降低和丙二醛（ MDA）含量的持续升高，且具有浓度依赖性；机理研究结果显示，浓度大于40μmol/L的CdCl2可引起BFO细胞出现典型的凋亡细胞形态，BFO细胞在AO/EB荧光双染色中的质膜通透性显著提高，在单细胞凝胶电泳中出现明显的彗尾，且细胞凋亡率、彗尾的长度和亮度随CdCl2浓度的增加而逐渐升高，并具有浓度依赖性。可见，镉对BFO细胞具有显著的毒性作用，其毒性作用是通过诱导细胞凋亡来实现的，为利用BFO细胞系研究镉等重金属的细胞毒性及其作用机理奠定了基础。
사용불동농도적록화력（ CdCl2）처리체외배양적조반성접란소（ BFO）세포계세포，이용독이학화세포생물학방법연구료중금속력대BFO세포적세포독성급기작용궤리。독성작용연구결과현시，BFO세포대CdCl2민감，농도대우10μmol/L적CdCl2대세포적생장구유현저적억제작용，능인기세포내초양화물기화매（ SOD）、곡광감태과양화물매（ GST-Px）활성적지속강저화병이철（ MDA）함량적지속승고，차구유농도의뢰성；궤리연구결과현시，농도대우40μmol/L적CdCl2가인기BFO세포출현전형적조망세포형태，BFO세포재AO/EB형광쌍염색중적질막통투성현저제고，재단세포응효전영중출현명현적혜미，차세포조망솔、혜미적장도화량도수CdCl2농도적증가이축점승고，병구유농도의뢰성。가견，력대BFO세포구유현저적독성작용，기독성작용시통과유도세포조망래실현적，위이용BFO세포계연구력등중금속적세포독성급기작용궤리전정료기출。
To investigate the cytotoxicity and its underlying mechanisms of cadmium chloride ( CdCl2 ) to barfin floun-der ovary ( BFO) cells, in vitro cultured BFO cells were treated with CdCl2 at different concentrations and investigated toxicologically and cytologically in this study.Cytotoxicity results showed that the BFO cells were very sensitive to CdCl2 , and their cell growth were inhibited in a concentration-dependent manner by CdCl2 at a concentration above 10μmol/L.CdCl2 at a concentration above 40μmol/L could reduce the activities of superoxide dismutase ( SOD) and glutathione peroxidase ( GST-Px), and elevate the content of melondialdehyde ( MDA) in BFO cells significantly. Mechanism results showed that typical morphological changes similar to those of apoptotic cells were found in BFO cells after treated with CdCl2 at a concentration above 40μmol/L.Obvious elevation of plasma membrane permeability in AO/EB double fluorescent staining and comet tails in single cell gel electrophoresis were both found in CdCl2 treated BFO cells.And the apoptotic ratio of BFO cells, the length and fluorescent intensity of comet tails from BFO cells were increased with the concentration of CdCl2 .In conclusion, CdCl2 has significant cytotoxicity to BFO cells which was achieved by inducing cell apoptosis, which has laid a solid foundation for studies of the cytotoxicity and toxic mecha-nisms of cadmium and the other heavy metals.