CAJ | 학술논문

以陇薯10号试管苗茎段为外植体，分别在5种培养基上进行愈伤组织诱导，比较接种后28 d时的出愈率及愈伤组织的形态，并将胚性愈伤接到10种不同分化培养基上进行再生苗分化。结果表明：接种28 d后，5种培养基均可诱导出大量的愈伤组织， MS+2.0 mg/L 6-BA+0.2 mg/L NAA+3.0 mg/L GA3+0.5 mg/L 2，4-D培养基上胚性愈伤诱导率最高，生长状态最好。胚性愈伤在MS+2.0 mg/L 6-BA+2.0 mg/L ZT+3.0 mg/L GA3培养基上分化率最高，且分化苗粗壮。
이롱서10호시관묘경단위외식체，분별재5충배양기상진행유상조직유도，비교접충후28 d시적출유솔급유상조직적형태，병장배성유상접도10충불동분화배양기상진행재생묘분화。결과표명：접충28 d후，5충배양기균가유도출대량적유상조직， MS+2.0 mg/L 6-BA+0.2 mg/L NAA+3.0 mg/L GA3+0.5 mg/L 2，4-D배양기상배성유상유도솔최고，생장상태최호。배성유상재MS+2.0 mg/L 6-BA+2.0 mg/L ZT+3.0 mg/L GA3배양기상분화솔최고，차분화묘조장。
To establish high efficient regeneration system of new potato (Solarium tuberosum L .) variety Longshu 10, stem segments of Longshu 10 tube seedling were inoculated on 5 kinds of culture media to induce callus. After 28 d from inoculation , the frequency of callus induction and callus morphology were compared. The embryonic callus was inoculated on 10 different differentiation media for differentiation of seedling . The results showed that after 28 d from inoculation, a lot of callus were induced from all 5 kinds of media . The frequency of embryonic callus from the medium MS+2 .0 mg/L 6-BA + 0.2 mg/L NAA + 3.0 mg/L GA3 +0.5 mg/L 2,4-D was highest, and the growth conditions of embryonic callus was best . The frequency of differentiated seedlings from the medium MS+2.0 mg/L 6-BA+2.0 mg/L ZT+3.0 mg/L GA3 was highest, and the differentiation stout seedlings was thick.