CAJ | 학술논문

建立了利咽颗粒中蝙蝠葛碱、蝙蝠葛苏林碱的含量测定方法。采用DiamonsilTMC18色谱柱（250 mm ×4.6 mm,5μm）,以乙腈-体积分数为0.05%三乙胺水溶液为流动相，流速为1.5 mL·min-1,检测波长为284 nm,进样量20μL,柱温为35℃。在上述色谱条件下,蝙蝠葛碱和蝙蝠葛苏林碱在1.106～8.848μg·mL-1(r=0.9995)、5.6～28μg·mL-1(r=0.9998)范围内线性关系良好,蝙蝠葛碱和蝙蝠葛苏林碱的平均加样回收率（n=9）均为98.7％；RSD分别为1.6%、1.5%。该法简便、准确、重复性好，可用于利咽颗粒中蝙蝠葛碱和蝙蝠葛林碱的含量测定。提高了利咽颗粒的质量控制标准，为其临床用药的安全性和有效性提供了质量保证。
건립료리인과립중편복갈감、편복갈소림감적함량측정방법。채용DiamonsilTMC18색보주（250 mm ×4.6 mm,5μm）,이을정-체적분수위0.05%삼을알수용액위류동상，류속위1.5 mL·min-1,검측파장위284 nm,진양량20μL,주온위35℃。재상술색보조건하,편복갈감화편복갈소림감재1.106～8.848μg·mL-1(r=0.9995)、5.6～28μg·mL-1(r=0.9998)범위내선성관계량호,편복갈감화편복갈소림감적평균가양회수솔（n=9）균위98.7％；RSD분별위1.6%、1.5%。해법간편、준학、중복성호，가용우리인과립중편복갈감화편복갈림감적함량측정。제고료리인과립적질량공제표준，위기림상용약적안전성화유효성제공료질량보증。
A HPLC method was established to determine contents of dauricine and daurisoline in Liyan Granules. Sample were analyzed on an DiamonsilTMC18(250 mm×4.6, 5 μm)column eluted with acetonitrile and water containing 0.05% triethy lamine as mobile phase in a gradient model; the flow rate was 1.5mL·min-1, the detective wavelength was set at 284 nm,and the column temperature was set at 35℃. The linear response ranges were 1.106~8.848μg·mL-1 (r=0.999 5) and 5.6~28μg·mL-1 (r=0.999 8) for dauricine and daurisoline ,respectively,The average recoveries (n=9) of these compounds all were 98.7%,and the RSDs were 1.8%and 1.5%, respectively. The method is simple,accurate and reproducible for determination of dauricine and dauri-soline in Liyan Granules simutaneously, and its quality standard can be improved, the method can provide a quality assurance for medicinal safety and clinical effectiveness of Liyan granules.