CAJ | 학술논문

壳聚糖（chitosan）及其降解产物因具有优良的物理特性和生物活性而被广泛关注。通过平板透明圈初筛、摇瓶复筛方法，从青岛海岸土壤中分离筛选到1株产壳聚糖酶活性较高的细菌Mitsuaria sp.K1，并对其产酶发酵条件进行了单因素试验和响应面优化分析试验。结果表明：在最适培养基组成（1%粉末壳聚糖、0.5%硝酸钾、0.22%KH2PO4、0.1%Na2HPO4、0.15%KCl、0.05%MgSO4·7H2O）和最佳培养条件（培养温度25.2℃，培养时间25.4 h，起始pH值6.5，接种量3%，装液量100 mL/500 mL摇瓶，160 r/min）下，Mitsuaria sp.K1的发酵粗酶液最高酶活平均达11.56 U/mL，比优化前的2.17 U/mL提高了4.32倍。与前人研究结果相比，该菌发酵产酶温度降低了5～10℃，产酶周期缩短了23～47 h，因此具有工业发酵应用价值。
각취당（chitosan）급기강해산물인구유우량적물리특성화생물활성이피엄범관주。통과평판투명권초사、요병복사방법，종청도해안토양중분리사선도1주산각취당매활성교고적세균Mitsuaria sp.K1，병대기산매발효조건진행료단인소시험화향응면우화분석시험。결과표명：재최괄배양기조성（1%분말각취당、0.5%초산갑、0.22%KH2PO4、0.1%Na2HPO4、0.15%KCl、0.05%MgSO4·7H2O）화최가배양조건（배양온도25.2℃，배양시간25.4 h，기시pH치6.5，접충량3%，장액량100 mL/500 mL요병，160 r/min）하，Mitsuaria sp.K1적발효조매액최고매활평균체11.56 U/mL，비우화전적2.17 U/mL제고료4.32배。여전인연구결과상비，해균발효산매온도강저료5～10℃，산매주기축단료23～47 h，인차구유공업발효응용개치。
Nowadays,people pay much attention to chitosan and its degradation product due to its excellent physical characteristic and biological activity. In this study,a high Chitosanase-producing bacterium Mitsuaria sp.K1 was isolated from the soil of Qingdao seacoast by primary screening of plate specula and shaking cultivation. The optimization of fermentation conditions was studied by Response Surface Methodology. The results showed that,under the optimal composition of medium (1.0%powder chitosan,0.5%KNO3,0.22%KH2PO4,0.1%Na2HPO4,0.15%KCl,0.05%MgSO4·7H2O) and the optimal cultural condition(25.2 ℃ incubating temperature,25.4 hours incubating time,initial pH6.5,3%inoculation amount,100 mL medium/500 mL flask,160 r/min),the maximal chitosanase activity of Mitsuaria sp.K1 could reach 11.56 U/mL which was 5.32 times of primary strain. In addition,due to Mitsuaria sp.K1 with lower temperature and shorter fermentation time of chitosanase production,it has a good potential in industrial fermentation.