CAJ | 학술논문

目的：比较在不同级别胚胎间人胰岛素样生长因子-Ⅱ（ IGF-Ⅱ）表达的不同，旨在从分子水平完善卵泡和胚胎发育的形态学评分标准，挑选出发育潜力更高的优质胚胎，提高体外受精-胚胎移植（ IVF-ET）的临床妊娠率。方法选取2013年2月～6月期间因单纯性输卵管阻塞行第一周期IVF-ET 患者31例，挑选正常受精的胚胎，收集其第3天移植后的胚胎培养液，ELISA法测其中IGF-Ⅱ的表达水平。结果不同级别胚胎间胚胎培养液中IGF-Ⅱ的分泌浓度：I级＞Ⅱ级＞Ⅲ级，且各组之间比较有统计学意义（P＜0．05），Ⅲ级与Ⅳ级间比较无统计学差异（ P＞0．05）。结论胚胎培养液中IGF-Ⅱ的定量检测有望在临床实践中成为选择胚胎的一个参数，作为辅助形态学评估选择胚胎移植，预测IVF的妊娠率。
목적：비교재불동급별배태간인이도소양생장인자-Ⅱ（ IGF-Ⅱ）표체적불동，지재종분자수평완선란포화배태발육적형태학평분표준，도선출발육잠력경고적우질배태，제고체외수정-배태이식（ IVF-ET）적림상임신솔。방법선취2013년2월～6월기간인단순성수란관조새행제일주기IVF-ET 환자31례，도선정상수정적배태，수집기제3천이식후적배태배양액，ELISA법측기중IGF-Ⅱ적표체수평。결과불동급별배태간배태배양액중IGF-Ⅱ적분비농도：I급＞Ⅱ급＞Ⅲ급，차각조지간비교유통계학의의（P＜0．05），Ⅲ급여Ⅳ급간비교무통계학차이（ P＞0．05）。결론배태배양액중IGF-Ⅱ적정량검측유망재림상실천중성위선택배태적일개삼수，작위보조형태학평고선택배태이식，예측IVF적임신솔。
Objective To compare the differences of IGF-Ⅱexpressions among the groups of the different grade procaryon and embryos .All of these are to find an easy ,no infringement and high precise method of embryo evaluation to improve the pregnancy rate of IVF . Methods Thirty-one cases of patients with simple tubal obstruction undergoing first treatment cycle of IVF -ET were enrolled.The embryo culture media after transplantation in the 3th day was selected ,IGF-Ⅱconcentrations were measured by ELISA assay .Results The IGF-Ⅱsecretion concentration of different levels of embryos in embryo culture media:Grade I >GradeⅡ>GradeⅢ >GradeⅣ,and the compari-sons among groups were statistically significant (P<0.05).Conclusion The quantitative detection of IGF-Ⅱin embryo cultures is expected to become embryo selection parameters ,to enhance embryo implantation rate .