CAJ | 학술논문

目的：观察IL-8、IL-1β、硫酸脱氢表雄酮（ DHEA-S）、前列腺素E2（ PGE2）对宫颈组织的作用机制。方法5组数量、体型、孕龄等均相同的孕白鼠分别受试于安慰剂或阴道栓剂（3次/d，连用3d）。分别应用人重组IL-8（100ng），IL-1β（200ng）， DHEA-S（10mg），PGE2（1mg）。白鼠均连续应用3d。宫颈坚实度、扩张度及含水量在末次给药24h后监测，用抗白鼠RT2抗体标记的宫颈组织行白细胞浸润检测，苏木苏-伊红染色后检测相关胶原浓度，胶原酶、凝胶酶及弹性蛋白酶的活性在100mg宫颈组织均浆中检测。结果含水量在所有受检宫颈组织中增加意义重大。 IL-8、IL-1β、前列腺素受试组的中性粒细胞数第3次给药后明显增加，硫酸脱氢表雄酮受试组的中性粒细胞数无明显改变。第1天用药后的IL-8，IL-1β和PGE2受试组胶原含量显著降低。第3天用药后，DHEA-S受试组胶原含量明显降低。2次用药后，除外对照组及前列腺素受试组，各组胶原酶活性均明显增高，所有受试组凝胶酶活性均增高。弹性蛋白酶同凝胶酶。结论白鼠宫颈的成熟由不同机制引发。细胞因子，如IL-8，IL-1β，DHEA-S，PGE2等在宫颈成熟中起重要作用。
목적：관찰IL-8、IL-1β、류산탈경표웅동（ DHEA-S）、전렬선소E2（ PGE2）대궁경조직적작용궤제。방법5조수량、체형、잉령등균상동적잉백서분별수시우안위제혹음도전제（3차/d，련용3d）。분별응용인중조IL-8（100ng），IL-1β（200ng）， DHEA-S（10mg），PGE2（1mg）。백서균련속응용3d。궁경견실도、확장도급함수량재말차급약24h후감측，용항백서RT2항체표기적궁경조직행백세포침윤검측，소목소-이홍염색후검측상관효원농도，효원매、응효매급탄성단백매적활성재100mg궁경조직균장중검측。결과함수량재소유수검궁경조직중증가의의중대。 IL-8、IL-1β、전렬선소수시조적중성립세포수제3차급약후명현증가，류산탈경표웅동수시조적중성립세포수무명현개변。제1천용약후적IL-8，IL-1β화PGE2수시조효원함량현저강저。제3천용약후，DHEA-S수시조효원함량명현강저。2차용약후，제외대조조급전렬선소수시조，각조효원매활성균명현증고，소유수시조응효매활성균증고。탄성단백매동응효매。결론백서궁경적성숙유불동궤제인발。세포인자，여IL-8，IL-1β，DHEA-S，PGE2등재궁경성숙중기중요작용。
Objective To study and compare the mechanism of action of interieukin (IL)-8,IL-1βdehydroepiandrosterone sul-phate and prostaglandin(PG)E2 on the cervix.Methods Five equal groups of pregnant mice were tested by either placebo or tested drugs in the form of vaginal suppositories once daily for 3 days.The suppositories contained human recombinant IL-8(100ng),IL-1β(200ng),DHEA-S(10mg) or PGE2(1mg).All mice were tested by one dose,two doses or three doses.Consistency,dilatation and water contents were esti-mated 24h after the last dose of treatment Leukocyte infiltration of the cervices was studied after staining the cervical tissue sections with anti -mouse RT2 monoclonal antibodies .Relative collagen concentration was assessed after staining with Picrosirius Red .Collagenase , gelatinase and elastase activities were measured in 100mg of homogenized cervical connective tissue .Results Water contents were significantly in-creased in all tested cervices .Neutrophil numbers were increased in IL-8 IL-1βgroups and PGE2 group after the third dose of treatment , whereas in DHEA-S group no significant changes were observed .Collagen content was significantly decreased in IL-8,EL-1βand PGE2 groups after the first dose of treatment .In the DHEA-S group,the decrease in collagen content occurred after the third dose .Collagenase activity was markedly increased in IL-8,IL-1β,and DHEA-S groups after the second dose of treatment .No significant increase in collagenase activity was found in PGE2 group.Gelatinase activity was significantly increased in IL-8,IL-1β,PGE2 and DHEA-S groups after the second dose of treat-ment.Also,elastase activity was increased after the second dose of treatment in all groups .Conclusion Our data suggests that ripening of the cervix in rabbit can be initiated by different mechanisms .Cytokines play a vital role in cervical ripening ,especially IL-8 and IL-1β.