CAJ | 학술논문

本试验旨在探究普安银鲫早期发育过程中消化酶活性变化及外源维生素C对其的影响。采用普安银鲫的成熟卵，以兴国红鲤（ Cyprinus carpio）进行异源人工干法授精，分为2组，孵化水体维生素C浓度分别为0和30 mg/L，分别在成熟卵、受精卵、原肠中期、晶体出现期、混合营养期及外源营养期采样，测定消化酶活性的变化。结果表明：1）维生素C能促进普安银鲫早期发育过程中蛋白质的沉积。2）成熟卵经异源授精后淀粉酶活性显著提高（ P＜0．05）。3）从原肠中期到外源营养期，胰蛋白酶和淀粉酶活性随发育呈上升趋势，各时期间差异均显著（P＜0．05）；胃蛋白酶、脂肪酶和纤维素酶活性从原肠中期到晶体出现期无显著上升（ P＞0．05），而在晶体出现期至外源营养期这3种酶活性呈上升趋势，各时期间差异均显著（ P＜0．05）。4）30 mg/L的维生素C溶液浸泡能显著提高胰蛋白酶、淀粉酶、脂肪酶和胃蛋白酶活性（ P＜0．05），但对纤维素酶活性无显著影响（ P＞0．05）。结果提示，普安银鲫成熟卵经异源授精后淀粉酶优先发挥作用，分解卵黄中的碳水化合物为胚胎发育迅速提供能量；胰蛋白酶、淀粉酶、胃蛋白酶和脂肪酶分解卵黄物质，在器官形成及仔鱼发育中起着主要作用；30 mg/L的维生素C能显著提高胰蛋白酶、淀粉酶、胃蛋白酶和脂肪酶活性，并促进蛋白质的沉积。
본시험지재탐구보안은즉조기발육과정중소화매활성변화급외원유생소C대기적영향。채용보안은즉적성숙란，이흥국홍리（ Cyprinus carpio）진행이원인공간법수정，분위2조，부화수체유생소C농도분별위0화30 mg/L，분별재성숙란、수정란、원장중기、정체출현기、혼합영양기급외원영양기채양，측정소화매활성적변화。결과표명：1）유생소C능촉진보안은즉조기발육과정중단백질적침적。2）성숙란경이원수정후정분매활성현저제고（ P＜0．05）。3）종원장중기도외원영양기，이단백매화정분매활성수발육정상승추세，각시기간차이균현저（P＜0．05）；위단백매、지방매화섬유소매활성종원장중기도정체출현기무현저상승（ P＞0．05），이재정체출현기지외원영양기저3충매활성정상승추세，각시기간차이균현저（ P＜0．05）。4）30 mg/L적유생소C용액침포능현저제고이단백매、정분매、지방매화위단백매활성（ P＜0．05），단대섬유소매활성무현저영향（ P＞0．05）。결과제시，보안은즉성숙란경이원수정후정분매우선발휘작용，분해란황중적탄수화합물위배태발육신속제공능량；이단백매、정분매、위단백매화지방매분해란황물질，재기관형성급자어발육중기착주요작용；30 mg/L적유생소C능현저제고이단백매、정분매、위단백매화지방매활성，병촉진단백질적침적。
This experiment was conducted to study the variation of digestive enzyme activities of Carassius au-ratus during early development and the effects of exogenous vitamin C.Mature eggs from Carassius auratus were selected and cross-fertilized using Cyprinus carpio by the method of artificial dried fertilization.The eggs were divided into two groups, and the vitamin C concentration in water for incubation was 0 and 30 mg/L, re-spectively.Samples were collected at mature eggs, fertilized eggs, mid-gastrula, crystal appear, mixed nutri-tion and exogenous nutrition stages to determine the variation of digestive enzyme activities.The results showed as follows:1) vitamin C was contributed to the deposition of protein in Carassius auratus during early devel-opment.2) After cross-fertilization, the activity of amylase was significantly higher than that of mature eggs ( P<0.05) .3) From mid-gastrula stage to exogenous nutrition stage, the activities of trypsin and amylase were increased with development, the differences among stages were significant ( P<0.05);the activities of pepsin, lipase and cellulase had no obvious rise from mid-gastrula stage to crystal appear stage ( P>0.05 ) , but were raised from crystal appear stage to exogenous nutrient stage, the differences were significant (P<0.05).4) The inhabitation in 30 mg/L vitamin C solution significantly increased the activities of trypsin, amylase, lipase and pepsin, but had no obvious effect on cellulase activity.The results indicate that amylase priority decompo-sition carbohydrates in yolk of mature eggs from Cyprinus carpio to provide energy for embryonic development rapidly after cross-fertilization;trypsin, amylase, pepsin and lipase play major roles in decomposition yolk nu-trients, organ formation and the development of larvae;30 mg/L vitamin C solution can significantly increase the activities of trypsin, amylase, pepsin and lipase, and elevate the deposition of protein.