中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2014年
5期
372-375
,共4页
干卓坤%张丽%刘秀华%李瑞生%龚美亮%周玉%张丽萍
榦卓坤%張麗%劉秀華%李瑞生%龔美亮%週玉%張麗萍
간탁곤%장려%류수화%리서생%공미량%주옥%장려평
血红素氧合酶-1%心力衰竭%炎症
血紅素氧閤酶-1%心力衰竭%炎癥
혈홍소양합매-1%심력쇠갈%염증
heme oxygenase-1%heart failure%inflammation
目的：研究血红素氧合酶-1（HO-1）对心力衰竭（心衰）大鼠肠道炎症的保护机制。方法通过冠状动脉结扎术造成心肌梗死建立心衰大鼠模型（雄性，Wistar大鼠），每组10只，分为心肌梗死（MI）模型组、MI＋钴-原卟啉（MI＋Copp）组、MI＋锡中卟啉（MI＋SnMP）组，以正常大鼠作为对照组，分别腹腔注射生理盐水、Copp溶液、SnMP溶液。8周后取门静脉及下腔静脉血检测血浆内毒素含量，通过Western印迹测定小肠HO-1的表达，比色法测定小肠一氧化碳（CO）浓度，酶联免疫吸附法测定小肠肿瘤坏死因子-α和白细胞介素-10水平。结果与MI组比较，MI＋Copp组HO-1和CO水平明显升高，血浆内毒素含量减少，肠道炎症减轻，而MI＋SnMP组HO-1无明显变化，CO明显降低，血浆内毒素浓度升高，小肠炎症加重。结论 HO-1可抑制心衰大鼠肠道炎症，该作用可能与CO有关。
目的：研究血紅素氧閤酶-1（HO-1）對心力衰竭（心衰）大鼠腸道炎癥的保護機製。方法通過冠狀動脈結扎術造成心肌梗死建立心衰大鼠模型（雄性，Wistar大鼠），每組10隻，分為心肌梗死（MI）模型組、MI＋鈷-原卟啉（MI＋Copp）組、MI＋錫中卟啉（MI＋SnMP）組，以正常大鼠作為對照組，分彆腹腔註射生理鹽水、Copp溶液、SnMP溶液。8週後取門靜脈及下腔靜脈血檢測血漿內毒素含量，通過Western印跡測定小腸HO-1的錶達，比色法測定小腸一氧化碳（CO）濃度，酶聯免疫吸附法測定小腸腫瘤壞死因子-α和白細胞介素-10水平。結果與MI組比較，MI＋Copp組HO-1和CO水平明顯升高，血漿內毒素含量減少，腸道炎癥減輕，而MI＋SnMP組HO-1無明顯變化，CO明顯降低，血漿內毒素濃度升高，小腸炎癥加重。結論 HO-1可抑製心衰大鼠腸道炎癥，該作用可能與CO有關。
목적：연구혈홍소양합매-1（HO-1）대심력쇠갈（심쇠）대서장도염증적보호궤제。방법통과관상동맥결찰술조성심기경사건립심쇠대서모형（웅성，Wistar대서），매조10지，분위심기경사（MI）모형조、MI＋고-원계람（MI＋Copp）조、MI＋석중계람（MI＋SnMP）조，이정상대서작위대조조，분별복강주사생리염수、Copp용액、SnMP용액。8주후취문정맥급하강정맥혈검측혈장내독소함량，통과Western인적측정소장HO-1적표체，비색법측정소장일양화탄（CO）농도，매련면역흡부법측정소장종류배사인자-α화백세포개소-10수평。결과여MI조비교，MI＋Copp조HO-1화CO수평명현승고，혈장내독소함량감소，장도염증감경，이MI＋SnMP조HO-1무명현변화，CO명현강저，혈장내독소농도승고，소장염증가중。결론 HO-1가억제심쇠대서장도염증，해작용가능여CO유관。
Objective To investigate the underlying mechanism through which heme oxygenase-1 (HO-1) protects intestine against inflammation in rats with heart failure. Methods Heart failure model was established in male Wistar rats by myocardial infarction (MI) with coronary ligation. These model rats were randomized into 3 experimental groups (10 rats in each group):Myocardial infarction (MI), MI+cobalt protoporphyrin(Copp), and MI+stannum+mesoporphyrin Ⅸ dichloride (SnMP;a HO-1 inhibitor) groups, receiving intra-peritoneal injection of saline, cobalt protoporphyrin solution, and stannum mesoporphyrin Ⅸ dichloride solution, respectively. Another 10 rats served as normal controls and received intraperitoneal injection of normal saline. After 8 weeks, the endotoxin level in portal vein and inferior vena cava, the expression of HO-1, and the contents of carbon monoxide (CO), tumor necrosis factor (TNF)-α, and interleukin-10 in the intestine were determined by Western blotting, colorimetry, and enzyme-linked immunosorbent assay (ELISA). Results Compared with MI group, MI+Copp group had significantly elevated HO-1 and CO expression, reduced endotoxin, milder inflammation in the intestine, and these benefits were abolished by SnMP, with obviously decreased CO, elevated endotoxin and severer intestinal inflammation. Conclusion HO-1 suppresses intestinal inflammation, which may be associated with CO.
