CAJ | 학술논문

目的：用不同种类液体对重度失血性休克大鼠进行复苏后，对比大鼠心肌组织中热休克蛋白（HSP）90α的表达、变化规律及大鼠生存率，为临床治疗失血性休克选择复苏液体提供参考。方法建立失血性休克大鼠模型，分别运用乳酸林格液、聚明胶肽注射液、高渗氯化钠右旋糖酐进行液体复苏，测定大鼠心肌组织中HSP90α蛋白的表达及大鼠死亡率。结果重度失血性休克后运用不同种类液体进行复苏，大鼠心肌组织HSP90α表达、大鼠死亡率差异有统计学意义（P＜0．05）。结论重度失血性休克可以诱导大鼠心肌组织中HSP90α的表达，当用不同液体进行复苏后各组HSP90α的表达不同且具有一定的规律性，提示HSP90α作为一个重要的调控分子参与失血性休克后心肌组织的保护机制。
목적：용불동충류액체대중도실혈성휴극대서진행복소후，대비대서심기조직중열휴극단백（HSP）90α적표체、변화규률급대서생존솔，위림상치료실혈성휴극선택복소액체제공삼고。방법건립실혈성휴극대서모형，분별운용유산림격액、취명효태주사액、고삼록화납우선당항진행액체복소，측정대서심기조직중HSP90α단백적표체급대서사망솔。결과중도실혈성휴극후운용불동충류액체진행복소，대서심기조직HSP90α표체、대서사망솔차이유통계학의의（P＜0．05）。결론중도실혈성휴극가이유도대서심기조직중HSP90α적표체，당용불동액체진행복소후각조HSP90α적표체불동차구유일정적규률성，제시HSP90α작위일개중요적조공분자삼여실혈성휴극후심기조직적보호궤제。
Objective To explore the expression changes of HSP90αin cardiac muscles and survival rates in rats by using the different fluids to resuscitate the severs hemorrhagic shocked rats ,and provide reference for the clinical treatment of hemorrhagic shock with different resuscitation fluids .Methods Uncontrolled hemorrhagic shock rats model was established ,using lactic acid salinger liquid ,poly peptide injection gelatin ,hypertonic sodium chloride dextran for fluid resuscitation respectively ,and then checked the HSP90αexpression changes and survival rates in rats .Results the expressions of HSP90αin myocardial tissue and the mortality in rats were different after using different resuscitation fluids in severe hemorrhagic shock rats ,difference was statistically significant(P<0 .05) .Conclusion the expression of HSP90α in cardiac muscles of rats could be induced by severe hemorrhagic shock ,the HSP90αexpressed differently and regularly after using different resuscitating fluids ,it implied that the HSP90α played an important role in the hemorrhagic rats cardiac as a regulating fator .