CAJ | 학술논문

目的：观察不同剂量的食用白酒对大鼠心脏结构和功能的影响，并探讨其对心脏损害的机制。方法将成年雄性SD大鼠54只分成生理盐水组、乙醇组和白酒组，每组各分为低、中和高剂量组。灌胃12周后，监测大鼠左心室内压，计算左心室内压变化幅度；测定全心质量指数；快速Masson染色观察心肌细胞纤维化情况；酶联免疫吸附试验（ELISA）检测心肌组织血管紧张素Ⅱ（AngⅡ）含量；荧光定量聚合酶链反应（PCR）法检测心肌组织血管紧张素转换酶（ACE）、ACE2 mRNA表达。结果白酒、乙醇增加了全心质量指数、左心室内压变化幅度，剂量依赖性地加重了心肌细胞变性，纤维组织增生，且剂量依赖性地增加了心肌组织AngⅡ含量及ACE mRNA表达。低剂量的白酒、乙醇增加了心肌组织ACE2 mRNA表达，但其中、高剂量降低了心肌组织ACE2 mRNA表达。结论长期饮酒可破坏心肌内的ACE和ACE2平衡，使ACE和AngⅡ升高，ACE2降低，导致心肌纤维化，其纤维化的变化程度与饮酒量以及时间长短有关。
목적：관찰불동제량적식용백주대대서심장결구화공능적영향，병탐토기대심장손해적궤제。방법장성년웅성SD대서54지분성생리염수조、을순조화백주조，매조각분위저、중화고제량조。관위12주후，감측대서좌심실내압，계산좌심실내압변화폭도；측정전심질량지수；쾌속Masson염색관찰심기세포섬유화정황；매련면역흡부시험（ELISA）검측심기조직혈관긴장소Ⅱ（AngⅡ）함량；형광정량취합매련반응（PCR）법검측심기조직혈관긴장소전환매（ACE）、ACE2 mRNA표체。결과백주、을순증가료전심질량지수、좌심실내압변화폭도，제량의뢰성지가중료심기세포변성，섬유조직증생，차제량의뢰성지증가료심기조직AngⅡ함량급ACE mRNA표체。저제량적백주、을순증가료심기조직ACE2 mRNA표체，단기중、고제량강저료심기조직ACE2 mRNA표체。결론장기음주가파배심기내적ACE화ACE2평형，사ACE화AngⅡ승고，ACE2강저，도치심기섬유화，기섬유화적변화정도여음주량이급시간장단유관。
Objective To investigate the effects of different dose of edible liquor on cardiac structure and function in rats ,and explore its underlying mechanisms .Methods 54 male SD rats were randomly divided into saline group ,alcohol group ,liquor group , each group were further divided into low-,middle-and high-dose groups .After 12 weeks of feeding ,left ventricular pressure was de-termined ,and left ventricular pressure amplitude and indexes of HW /BW were calculated .Myocardial fibrosis was observed by Masson staining ,AngⅡ content of myocardial tissue were detected by ELISA ,and ACE and ACE2 mRNA expressions were detec-ted by RT-PCR ,respectively .Results Left ventricular pressure amplitude and HW/BW index were increased by both liquor and al-cohol .The myocardial cell turbidity ,degeneration ,hyperplasia of fibrous tissue change were gradually worsened ,and the AngⅡ con-tents and the ACEmRNA expressions of the myocardial tissue were also increased by both liquor and alcohol .The ACE2 mRNA ex-pression of the myocardial tissue were higher by low-dose liquor and alcohol ,while the ACE2 mRNA expression of the myocardial tissue were lower by middle-and high-dose liquor and alcohol .Conclusion Long-term alcohol consumption can damage the balance of cardiac ACE and ACE2 ,causing the increase of ACE ,AngⅡ and the decrease of ACE2 ,resulting in myocardial fibrosis ,whose degree is related to the amount of alcohol consumption and drinking history .