CAJ | 학술논문

目的：建立维生素D缺乏大鼠模型，并进行初步评价，为研究维生素D缺乏与高血压的关系奠定基础。方法将成年的SD大鼠分成正常对照组、维生素D缺乏组和维生素D对照组，分别采用普通正常饲料、维生素D缺乏饲料和维生素D对照饲料进行饲养，饲养环境采用白炽灯作为照明光源，维持12 h：12 h光暗周期；饲养6周后通过眼底取血的方式采集外周血，使用EIA法测定血清维生素D含量，之后每隔两周测定1次。结果饲养10周后维生素D缺乏组大鼠血浆维生素D平均含量降至2．07 nmol/L ；维生素D对照组为56．74 nmol/L ，正常对照组为57．61 nmol/L ，正常对照组与维生素D对照组差异无统计学意义（P＞0．05）。与其余两组相比，维生素D缺乏组大鼠血压显著升高。结论通过控制光照和特殊饲料饲养方法建立维生素D缺乏大鼠模型是可行的，相比对照组，维生素D缺乏动物的血压显著升高。
목적：건립유생소D결핍대서모형，병진행초보평개，위연구유생소D결핍여고혈압적관계전정기출。방법장성년적SD대서분성정상대조조、유생소D결핍조화유생소D대조조，분별채용보통정상사료、유생소D결핍사료화유생소D대조사료진행사양，사양배경채용백치등작위조명광원，유지12 h：12 h광암주기；사양6주후통과안저취혈적방식채집외주혈，사용EIA법측정혈청유생소D함량，지후매격량주측정1차。결과사양10주후유생소D결핍조대서혈장유생소D평균함량강지2．07 nmol/L ；유생소D대조조위56．74 nmol/L ，정상대조조위57．61 nmol/L ，정상대조조여유생소D대조조차이무통계학의의（P＞0．05）。여기여량조상비，유생소D결핍조대서혈압현저승고。결론통과공제광조화특수사료사양방법건립유생소D결핍대서모형시가행적，상비대조조，유생소D결핍동물적혈압현저승고。
Objective To establish and evaluate animal model of adult rats with vitamin D deficient and assess the association of vitamin D deficiency and hypertension .Methods Adult SD rats were divided into 3 groups :normal controls group(feed with ordina-ry forage) ,vitamin D deficient groups(feed without vitamin D)and vitamin D control group(feed with vitamin D) ,which were fed with different diets under 12 h:12 h without ultraviolet light bulb(UV-B) .After treatment for 6 weeks ,Retinal blood was collect-ed ,and Serum vitamin D levels was tested by EIA every 2 weeks .Results After treatment for 10 weeks ,Serum level of vitamin D in vitamin D deficient group were reduced to 2 .07 nmol/L ,while vitamin D group and normal control group were measured as 56 .74 nmol/L and 57 .61 nmol/L ,there was no statistically significant difference between vitamin D group and normal control group (P>0 .05) .hypertension of vitamin D deficient group was significantly higher than other two groups .Conclusion It is feasible to estab-lish model of adult rats with vitamin D by UV-B free light and diets control ,hypertension of rats vitamin D deficiency has a signifi-cant rise compared with the control group .